枸杞多糖和AG490联用对宫颈癌细胞增殖和凋亡的影响研究  

The Effects of Lycium Barbarum Polysaccharides Combined with AG490 on the Proliferation and Apoptosis of Cervical Cancer Cells

作　　者：张崇媛 胡萍[1] 何秋敏[1] 王文容 张水蓉[1] 

机构地区：[1]荆州市中心医院妇产科,湖北荆州434020 [2]荆州市第三人民医院妇产科,湖北荆州434000

出　　处：《肿瘤药学》2017年第2期183-188,共6页Anti-Tumor Pharmacy

摘　　要：目的探讨枸杞多糖和AG490联用对宫颈癌细胞增殖凋亡的影响。方法 CCK8实验检测5、10、20、40、80mg·L^(-1)枸杞多糖及6.25、12.5、25、50、100μmol·L^(-1)的AG490作用于人宫颈癌HeLa细胞24、48、72 h后细胞的增殖情况,计算IC50值;根据IC50值选择最佳的枸杞多糖和AG490作用浓度;将试验分为对照组、枸杞多糖组、AG490组、枸杞多糖+AG490组,CCK8试验检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot检测Cleaved Caspase 3、JAK2、STAT3、p-STAT3蛋白表达。结果枸杞多糖和AG490均能显著抑制人宫颈癌HeLa细胞增殖,并呈时间和剂量依赖性(P<0.01),选择40 mg·L^(-1)枸杞多糖和70μmol·L^(-1) AG490进行后续研究;枸杞多糖组、AG490组、枸杞多糖+AG490组细胞抑制率、细胞凋亡率、Cleaved Caspase 3蛋白表达均显著高于对照组,JAK2、p-STAT3蛋白表达显著低于对照组(P<0.01);枸杞多糖+AG490组细胞抑制率、细胞凋亡率、Cleaved Caspase 3蛋白表达显著高于枸杞多糖组和AG490组,JAK2、p-STAT3蛋白表达显著低于枸杞多糖组和AG490组(P<0.01)。结论枸杞多糖和AG490均能抑制人宫颈癌HeLa细胞增殖,促进细胞凋亡,二者联用对细胞增殖和凋亡的影响强于单独使用枸杞多糖和AG490。Objective To investigate the effects of Lycium barbarum polysaccharides combined with AG490 on the proliferation and apoptosis of cervical cancer ceils. Methods Proliferation of human cervical carcinoma HeLa cells were detected after treatment of 5, 10, 20, 40, 80 mg·L^-1 LBP and 6.25, 12.5, 25, 50, 100 μmol·L^-1AG490 for 24, 48, 72 h by CCK-8 assay. ICs0 value was calculated, and the op- timum concentration of LBP and AG490 depended on ICs0. The HeLa cells were divided into control group, LBP group, AG490 group, LBP ＋ AG490 group. Cell proliferation was detected by CCK-8 assay, and apoptosis was detected by flow cytometry. The expressions of Cleaved Caspase 3, JAK2, STAT3 and p-STAT3 protein was detected by Western blot. Results LBP and AG490 both could significantly inhibit the proliferation of human cervical cancer HeLa cells in a dose and time dependent manner （P 〈 0.01）. 40 mg·L^-1 LBP and 70μmol·L^-1 AG490 were selected for the follow study. The cell proliferation inhibitory rate, apoptosis rate and Cleaved Caspase 3 protein expression were sig- nificantly higher in LBP group, AG490 group and LBP＋AG490 group than in control group, while the expression of JAK2 and p-STAT3 protein was significantly lower than in control group （P〈0.01）. The cell inhibitory rate, apoptosis rate and Cleaved Caspase 3 protein expres- sion in LBP＋AG490 group was significantly higher and the expression of JAK2 and p-STAT3 protein lower than in LBP group and AG490 group （P 〈 0.01）. Conclusion Both the LBP and AG490 could inhibit the proliferation of human cervical cancer HeLa cells and promote the apoptosis of cells. Additionally, the effect of the combination of two drugs was stronger than that of the single use of the LBP or AG490 on the proliferation and apoptosis of the HeLa cells.

关 键 词：枸杞多糖 AG490 宫颈癌 增殖 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]