树鼩经甲型肝炎减毒活疫苗、乙型肝炎疫苗及联合硫酸乙酰肝素佐剂免疫后的免疫效果评价  

The Immunity Effect Evluation on Tree Shrew by Hepatitis A Virus Attenuated Live Vaccine,Hepatitis B Vaccine Independently or Combined Adjuvant Heparan Sulfate

作　　者：王东宝[1] 胡云章[1] 胡凝珠[1] 罕园园[1] 匡德宣[1] 李彦涵[1] WANG Dong-bao HU Yun-zhang HU Ning-zhu HAN Yuan-yuan KUANG De-xuan LI Yan-han(Institute of Medical Biology, Chinese Academy of Medical College and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research ＆ Development on Severe Infectious Diseases, Yunnan Research Center of Engineering Technique for Vaccines against Severe Infectious Diseases, Yunnan Key Laboratory of Prevention ＆ Control Research on Insect-Borne Infectious Diseases, Kunming 650118, China)

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所疫苗研究室,云南省重大传染病疫苗研发重点实验室,云南省重大传染病疫苗工程技术研究中心,云南省虫媒传染病防控研究重点实验室,昆明650118

出　　处：《实验动物与比较医学》2017年第2期113-117,共5页Laboratory Animal and Comparative Medicine

基　　金：国家科技支撑计划项目(2014BAI01B01);国家重点研究计划生物安全关键技术研究发重点专项(2016YFC1202300)

摘　　要：目的探讨甲型肝炎减毒活疫苗(HepA-1)、乙型肝炎疫苗(Hbv)以及HepA-1、Hbv分别与佐剂硫酸乙酰肝素(HS)联合对树鼩进行免疫后树鼩体液免疫应答的影响。方法按照疫苗种类、佐剂以及免疫途径将树鼩随机分组,同时设空白对照组,分别于末次免疫后的4周、8周、12周、16周和20周尾静脉采血,用ELISA法检测树鼩血清中的特异性IgG抗体水平。结果除空白对照组外,实验组均在末次免疫的第四周检测到抗甲型肝炎病毒(HAV)及抗乙型肝炎病毒(HBV)抗体,抗体水平随着免疫时间延长而逐渐升高并于12周达到峰值,此后逐渐下降。同时,联合HS佐剂组的体液免疫效果均优于单纯疫苗免疫组。并且不同抗原以及联合佐剂采用不同的免疫途径免疫树鼩其免疫效果存在显著性差异。结论通过对树鼩进行疫苗免疫原性和增强免疫效果检测,证实以树鼩作为动物模型进行疫苗评价的可行性。Objective To investigate the humoral immune response of tree shrew after the immuni- zation of Hepatitis A attenuated live vaccine （HepA-1）, Hepatitis B vaccine （Hbv） independently and combined with adjuvant heparan sulfate （HS）. Methods The tree shrews were randomly divided into 10 groups by the type of vaccine, adjuvant and schedules. The specific IgG levels in sera of tree shrew were determined by ELISA at 4, 8, 12, 16 and 20 weeks after the last immunization respectively. Results Except the blank control group, the anti - hepatitis A virus （HAV） and anti - hepatitis B virus （HBV） antibody was detected in all the experimental group at 4 weeks after last immunization, the level of immune antibody were gradually increased with time and reached the peak at the 12 weeks, then decreased gradually. At the same time, the humoral immunity effect of HS adjuvant group were better than that of vaccine group. There is a significant difference on immunity among tree shrews with different antigens and adjuvant by different immunization schedules. Conclusion The immune effect of vaccine immunogenicity and immune enhancing effect on tree shrew confirms the feasibility of the tree shrew as an animal model.

关 键 词：树鼩 甲型肝炎减毒活疫苗(HepA—1) 乙型肝炎疫苗(Hbv) 硫酸乙酰肝素(HS) 体液免疫 

分 类 号：R-332[医药卫生] R392-33