香烟烟雾法肺动脉高压小鼠模型肺组织HIF-1α和Nrf2表达  

The Expression of HIF-1α and Nrf2 in the Lung of Pulmonary Arterial Hypertension Mice

作　　者：陈云芳[1] 王胜[2] 钱自亮[3] 

机构地区：[1]北京大学深圳研究生院化学院,广东深圳518055 [2]广州医科大学附属第一医院研究所广州呼吸疾病研究所,广东广州510120 [3]中山大学第八附属医院,广东深圳518033

出　　处：《江西师范大学学报（自然科学版）》2017年第2期155-159,共5页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(30971166);深圳市科技计划基础研究(JCYJ20140416094256880)资助项目

摘　　要：以香烟烟雾熏烟法建立肺动脉高压(PAH)小鼠模型,将C57B6J小鼠随机分为对照组和模型组,每组10只,对照组小鼠不作任何处理,模型组采用香烟处理:每天2次,每次2 h,10支/h,6 d/周,连续6个月的建模方法;使用小鼠右心室压力测定仪(BIOPAC Systems,MP150)测定小鼠右心室压力(RVSP);将肺小动脉进行胶原纤维染色(Van-Gieson法),结合Image Pro Plus软件分析,测量肺小动脉血管的中层壁厚和肺小动脉血管的外径,计算肺小动脉厚度的变化;利用Western Blot法检测Nrf2和HIF-1α蛋白在肺组织的表达.结果表明:1)与对照组相比,模型组小鼠右心室压力明显增加,肺小动脉血管壁明显增厚,管腔变狭窄,右心室与体重的比值显著增加;2)与对照组相比,模型组小鼠肺组织HIF-1α蛋白表达明显增加,而Nrf2蛋白表达明显减少.研究结果意味着:HIF-1α与Nrf2在香烟烟雾法建立的肺动脉高压小鼠肺组织中表达异常.Use cigarette smoke to establish pulmonary hypertension（PAH） mice model,C57B6 J mice were randomly divided into control group and model group,each group of 10,control group don’t make any processing,model group deal with cigarette：2 times a day,every time 2 h,10 piece/h,6 days/week,stick to 6 months to establish model;Use the mouse right ventricular pressure tester（BIOPAC Systems,MP150） measure right ventricular pressure（RVSP） of mice; Use pulmonary arteriole make collagen fiber staining（Van Gieson method）,combined with the Image Pro Plus software analysis,Measure pulmonary small arteries middle wall thickness and pulmonary small artery diameter,calculate the thickness of pulmonary arterioles change; Use Western Blot method to detect Nrf2 and HIF-1α protein expression in lung tissue.The results showed that：1） Compared with control mice,the right ventricular systolic pressure was increased significantly,the thickening of lung small artery wall and pulmonary stenosis were occurred,and the ratio of right ventricular/weight was significantly increased in model mice.2） The expression of HIF-1α was significantly increased,while the in the expression of Nrf2 was significantly reduced in model mice compared with control mice.The results mean：HIF-1α and Nrf2 had an abnormal expression in PAH mice.

关 键 词：肺动脉高压 吸烟 低氧诱导因子1Α 转录因子NF-E2相关因子2 

分 类 号：Q813[生物学—生物工程] R73-3[医药卫生—肿瘤]