天冬多糖对低氧条件下人肝癌细胞生长、侵袭及迁移的影响  

Effects of Asparagus polysaccharides on growth,invasion and migration of human hepatocellular carcinoma cells under hypoxia

作　　者：丁婕妤 王璇[1] 翁苓苓 李琦[2] 张闽光[1] 邢东炜[1] 

机构地区：[1]上海中医药大学附属市中医医院,上海200071 [2]上海中医药大学附属曙光医院

出　　处：《山东医药》2017年第14期23-26,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81673743);上海市自然科学基金资助项目(15ZR1438900);上海市博士点基金资助项目(b201404)

摘　　要：目的观察天冬多糖对低氧条件下人肝癌细胞生长、侵袭及迁移能力的影响,探讨天冬多糖的抗肿瘤作用机制。方法培养人肝癌细胞HepG2细胞,在96孔培养板中分别加入天冬多糖500、1 000、1 500、2 000、3 000、4 000μg/m L,另设空白对照孔加入生理盐水,放入低氧培养箱(95%N2,5%CO_2,1%O_2)继续培养,培养24、48、72 h时采用CCK-8法观察并计算细胞的抑制率。按照天冬多糖IC_(50)、IC_(20)、IC_(10)分别干预HepG2细胞,作为天冬多糖高浓度组、天冬多糖中浓度组、天冬多糖低浓度组,空白对照组不干预。各组放入低氧培养箱培养24 h后,采用Transwell法检测各组HepG2细胞的迁移及侵袭能力。结果不同浓度、不同作用时间的天冬多糖对HepG2细胞均有明显的生长抑制作用。相关性分析示,细胞生长抑制率与天冬多糖浓度呈正相关(r=0.980,P<0.05),具有剂量依赖性。天冬多糖低浓度组、天冬多糖中浓度组、天冬多糖高浓度组的侵袭及迁移细胞数均低于空白对照组(P均<0.01)。结论天冬多糖对低氧条件下人肝癌细胞的生长有明显的抑制作用,并能有效抑制人肝癌细胞的侵袭与迁移。Objective To observe the effect of Asparagus polysaccharides on the growth,invasion and migration of human hepatocellular carcinoma cells under hypoxia condition,and to explore the anti-tumor mechanism of Asparagus polysaccharides. Methods We cultured human hepatocellular carcinoma HepG2 cells,and added 500,1 000,1 500,2 000,3 000 and 4 000 μg/m L Asparagus polysaccharides to the 96-well culture plate. The control wells were added with the normal saline,then were put into the hypoxia incubator（ 95% N2,5% CO2,1% O2） for 24,48 and 72 hours. CCK-8 assay was used to detect the inhibition rate of Asparagus polysaccharides on human hepatocarcinoma cells. According to the Asparagus polysaccharides IC50,IC20,and IC10,we used the high-,medium-and low-concentrations of Asparagus polysaccharides to intervene HepG2 cells,which were then taken as the H-Asp,M-Asp,and L-Asp groups. The blank control group was not treated. After 24 h in hypoxia incubator,Transwell assay was used to detect the invasion and migration abilities of HepG2 cells. Results At different concentrations and different time,Asparagus polysaccharides had significant growth inhibition on HepG2 cells. The correlation analysis showed that the cell growth inhibition rate was positively correlated with the concentrations of Asparagus polysaccharides（ r = 0. 980,P〈 0. 05） with a dose-dependent manner. The number of invasive and migratory cells in the H-Asp,M-Asp,L-Asp groups was significantly less than that of the blank control group（ all P〈 0. 01）. Conclusion Asparagus polysaccharides can significantly inhibit the growth of human hepatocellular carcinoma cells under hypoxia as well as effectively inhibit their invasion and migration.

关 键 词：天冬多糖 低氧 肝癌 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤] R730.231[医药卫生—临床医学]