血浆内毒素及(1-3)-β-D葡聚糖测定在急性胰腺炎感染中的应用价值  

Application value of lipopolysaccharides and (1-3)-β-D glucan detection in acute pancreatitis complicating infection

作　　者：宋月娟[1] 冯强生[1] 哈小琴[1] 

机构地区：[1]兰州军区兰州总医院检验科,兰州730050

出　　处：《国际检验医学杂志》2017年第9期1163-1164,共2页International Journal of Laboratory Medicine

基　　金：甘肃省青年科技基金(145RJYA320)

摘　　要：目的研究急性胰腺炎(AP)患者血液中内毒素、(1-3)-β-D葡聚糖浓度检测的临床应用价值。方法采用MB-80微生物快速动态检系统检测AP患者血浆中的内毒素和(1-3)-β-D葡聚糖浓度,并进行分析。结果 184例AP合并胰腺周围脓肿和双肺感染等的患者,内毒素(G-脂多糖)测定阳性者23例,平均值为93.22pg/mL,阳性率为10.87%,经微生物培养证实其中15例为革兰阴性菌感染。175例AP合并胰腺周围脓肿和双肺感染等的患者中,检出67例(1-3)-β-D葡聚糖阳性,平均值为394.07pg/mL,阳性率为38.29%,与非AP患者组血清(1-3)-β-D葡聚糖阳性率比较,差异有统计学意义(P<0.05),经微生物培养或涂片,证实其中22例为真菌感染。结论血浆内毒素、(1-3)-β-D葡聚糖定量测定,为早期AP全身感染预测提供了实验室生物学指标,有助于AP感染严重程度评估和临床合理用药。Objective To investigate the clinical application value of endotoxin and(1-3)-β-D glucan detection in the patients with acute pancreatitis(AP).Methods The MB-80 microbiology rapid dynamic detection system was applied to detect the concentration of plasma LPS and(1-3)-β-D glucan in the patients with AP.Then the results were analyzed.Results Among 184 cases of AP complicating peripancreatic abscess and bilateral lung infection,endotoxin G-lipopolysaccharides positive was in 23 cases,the average value was 394.07pg/mL,the positive rate was 10.87%,and 15 cases of Gram-negative bacterial infection were verified by the microorganism culture.Among 175 cases of AP complicating peripancreatic abscess and bilateral lung infection,67 cases were(1-3)-β-D glucan positive,the average value was 93.22pg/mL,the positive rate was 38.29%,which were with the positive rate of serum(1-3)-β-D glucan in the non-AP group,the difference was statistically significant(P<0.05),the microorganism culture or smear verified that 22 cases were fungal infection.Conclusion Plasma endotoxin and(1-3)-β-D glucan detection provides the laboratory biological indicator and is conducive to the assessment of AP infection severity and clinical medication.

关 键 词：急性胰腺炎 内毒素 (1-3)-β-D葡聚糖 

分 类 号：R576[医药卫生—消化系统] R446.1[医药卫生—内科学]