大鼠肌肉挥发性有机化合物变化规律与死亡时间的关系  

Relationship between the Change Rules of Volatile Organic Compounds in Rat Muscle and Postmortem Interval

作　　者：刘蓓蓓[1] 夏志远[1] 马锦琦[1] 李朴[1] 吕坪[1] 周海梅[1] LIU Bei-bei XIA Zhi-yuan MA Jin-qi LI Pu LU Ping ZHOU Hai-mei(School of Forensic Medicine,Henan University of Science and Technology, Luoyang 471003, China)

机构地区：[1]河南科技大学法医学院,河南洛阳471003

出　　处：《法医学杂志》2017年第2期120-124,共5页Journal of Forensic Medicine

基　　金：河南省教育厅重点科研基金资助项目(16A150030);河南科技大学研究生创新基金资助项目(CXJJ-ZR22)

摘　　要：目的探究大鼠死后肌肉挥发性有机化合物(volatile organic compound,VOC)的变化规律与死亡时间(postmortem interval,PMI)的关系。方法健康大鼠120只,随机分成12组,每组10只,以颈椎脱臼方式处死后保存在(25±1)℃环境中,在死后即刻(0 d)、0.5、1、2、3、4、5、6、7、8、9、10 d共12个时间点分别取一组大鼠肌肉,采用顶空固相微萃取气质联用法收集、检测、分析大鼠肌肉VOC。结果共鉴定出15种VOC,其中芳香类9种、含硫类3种、脂肪酸类2种、杂环类1种。随PMI延长,VOC种类总数呈增加趋势,第1天没有检测到VOC,第2天检出3种,第3天检出9种,第4天检出11种,5~7 d检出14种,8~10 d检出15种。不同VOC峰面积与PMI存在一定的相关性(校准R^2=0.15~0.96),15种VOC总峰面积(y)与PMI(x)相关:2~5 d,回归方程为y=-17.05 x^2+164.36 x-246.36(校准R^2=0.96);6~10 d回归方程为y=2.24 x+101.13(校准R^2=0.97)。结论肌肉VOC变化规律有助于PMI的推断。Objective To explore the relationship between the change rules of volatile organic compounds （VOCs） in rat muscle and postmortem interval （PMI）. Methods A total of 120 healthy rats were divided randomly into 12 groups （10 for each group）. After the rats were sacrificed by cervical dislocation, the bodies were kept at （25±1） ℃. Rat muscle samples were separately obtained at 12 PMI points,including 0, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 d. The VOCs in rat muscles were collected, detected and ana-lyzed by headspace solid-phase micro extraction （HS-SPME） coupled to gas chromatography-mass spec-trometer （GC-MS）. Results In total, 15 species of VOCs were identified, including 9 aromatic com-pounds, 3 sulfur compounds, 2 aliphatic acids and 1 heterocyclic compound. The species of VOCs in-creased with PMI： no species were detected within 1 day, 3 species were detected on day 2, 9 on day 3,11 on day 4,14 from day 5 to 7,and 15 from day 8 to 10. Total peak area of 15 species of VOCs was significantly correlated to PMI （adjusted R2=0.15-0.96） ： the regression function was y=-17.05 x2＋ 164.36x-246.36 （adjusted R2=0.96） from day 2 to 5, and y=2.24x＋101.13 （adjusted R2=0.97） from day 6 to 10. Conclusion The change rules of VOCs in rat muscle are helpful for PMI estimation.

关 键 词：法医病理学 挥发性有机化合物 气相色谱-质谱法 固相微萃取 死亡时间 肌 大鼠 

分 类 号：R122.1[医药卫生—环境卫生学]