白杨素对高糖损伤血管内皮的影响  

Effects of chrysin on impaired vascular endothelial function induced by high glucose

作　　者：张夏丽[1] 韩斯嘉 罗乐[1] 陈敏[1] 李洁[1] 田晴晴[1] 高雪梅[2] 张轩萍[1] ZHANG Xia-li HAN Si-jia LUO Le CHEN Min LI Jie TIAN Qing-qing GAO Xue-mei ZHANG Xuan-ping(Dept of Pharmacology, Shanxi Medical University, Taiyuan 030001, China Dept of Physiology,Shanxi Medical College for Continuing Education, Taiyuan 030001, China)

机构地区：[1]山西医科大学药理学教研室,山西太原030001 [2]山西职工医学院生理学教研室,山西太原030001

出　　处：《中国药理学通报》2017年第5期707-712,共6页Chinese Pharmacological Bulletin

基　　金：山西省卫生科研资助项目(No 2015109)

摘　　要：目的探讨急性高糖条件下,白杨素(chrysin,Ch)对血管内皮舒张功能的影响。方法 (1)采用离体血管环实验,设立正常对照组(control)、白杨素处理组(Ch),观察正常糖浓度时,白杨素对大鼠离体主动脉的舒张作用;设立正常糖浓度组(NG,11 mmol·L^(-1) Glu)、高糖实验组(HG,44 mmol·L^(-1) Glu)、甘露醇对照组(M,11 mmol·L^(-1) Glu+33 mmol·L^(-1) Man)和白杨素急性给药组(HG+Ch,44 mmol·L^(-1) Glu+chrysin 1.0μmol·L^(-1) ),观察急性高糖孵育后,白杨素对大鼠离体主动脉舒张功能的影响。(2)采用HUVEC细胞系,设立正常糖浓度组(NG,5.5 mmol·L^(-1) Glu)、高糖实验组(HG,33.3 mmol·L^(-1) Glu)、甘露醇对照组(M,5.5 mmol·L^(-1) Glu+27.8 mmol·L^(-1) Man)以及不同浓度白杨素处理组(Ch 25、50μmol·L^(-1) ),观察白杨素对高糖孵育HUVEC细胞存活率的影响,并检测各组NO释放量。结果 (1)正常糖浓度时,白杨素能浓度依赖性地舒张大鼠主动脉环,其Emax和EC50为(58.94±9.61)%、51.9μmol·L^(-1) (P<0.01);高糖浓度时,HG组ACh诱导的血管舒张反应性明显降低,其Emax与EC50值分别下降至(32.12±3.92)%、78.0μmol·L^(-1) (P<0.01),但硝普钠(SNP)诱导的血管舒张无变化;白杨素急性干预后,可以明显改善高糖对ACh诱导的血管内皮依赖性舒张功能的损伤,其Emax与EC50值分别为(70.7±3.87)%和0.852μmol·L^(-1) (P<0.01)。(2)HUVEC高糖处理后,细胞存活率降低至(82.56±3.97)%,白杨素(25、50μmol·L^(-1) )干预后能浓度依赖地增强HUVEC的细胞存活率,其细胞存活率可分别增至(103.4±4.78)%、(107.6±11.58)%;并且能提高高糖处理后细胞培养液NO的释放量。结论白杨素可以保护急性高糖损伤的血管内皮舒张功能,并增加内皮NO释放。Aim To explore the effects of chrysin on endothelial dysfunction induced by acute high glucose.Methods ① The effects of chrysin on normal isolated aortic at contraction induced by PE and on endothelial dysfunction induced by high glucose were tested in the following medium： normal group, chrysin group; normal-glucose group： glucose 11mmol·L^-1 in Krebs＇ solution; high-glucose group： glucose 44 mmol·L^-1 in Krebs＇ solution; mannitol group： mannitol 33 mmol·L^-1 in Krebs＇ solution and chrysin group： 44 mmol·L^-1 Glu＋chrysin 1.0 μmol·L^-1 in Krebs＇ solution.② The effects of chrysin on HUVEC cell viability after incubated in high glucose were observed in the following groups： normal-glucose group： glucose 5.5 mmol·L^-1 in culture solution; high-glucose group： glucose 33.3 mmol·L^-1 in culture solution; mannitol group： mannitol 27.8 mmol·L^-1 in culture solution and chrysin group： chrysin（25,50 μmol·L^-1）in culture solution.And the NO release was also testd in these groups.Results ① Chrysin could induce vasodilation in a dose-dependent manner at normal glucose.The Emax was（58.94±9.61）%,and the EC50 value was 51.9 μmol·L^-1.After incubating the aortic rings with high glucose（44 mmol·L^-1）for 4 h, there were significant differences in ACh-induced vascular relaxation between the normal glucose group and the high glucose group.The Emax was（32.12±3.92）% and the EC50 value was 78.0 μmol·L^-1 of high glucose group（P〈0.01）.The endothelium-independent relaxation induced by SNP was not significantly different between the two groups.And chrysin（1.0 μmol·L^-1）could reverse the decline of ACh-induced vasorelaxation response induced by high glucose（44 mmol·L^-1）.The Emax was（70.7±3.87）% and the EC50 value was 0.852 μmol·L^-1.② The cell viability of HUVEC was depressed after incubated in high glucose, and chrysin could reverse the decline in a concentration-dependent way.And chrysin in defferent concentrations could increase the cell NO r

关 键 词：大鼠离体主动脉 血管环 白杨素 急性高糖 HUVEC NO 

分 类 号：R-332[医药卫生] R282.71