牛磺酸对实验性肝纤维化大鼠保护作用的研究  

Protective effects of Taurine on the experimental hepatic fibrosis rats

作　　者：李静[1] 杨雅娟[1] 冯艳[2] 李良宵 申军华[1] 董魁[1] 郭建华[1] LI J ing YANG Ya-juan FENG Yan LI Liang-xiao SHEN Jun -h ua DONG Kui GUO Jian -hua(Department ofGastroenterology, First Hospital ofHandan, Handan 056002, China Department ofLaboratory, First Hospital o f Handan, Handan 056002, China Department ofInfectious Diseases, First Hospital of Handan, Handan 056002, China)

机构地区：[1]邯郸市第一医院消化内科,邯郸056002 [2]邯郸市第一医院检验科,邯郸056002 [3]邯郸市第一医院感染性疾病科,邯郸056002

出　　处：《天津中医药》2017年第5期336-340,共5页Tianjin Journal of Traditional Chinese Medicine

摘　　要：[目的]研究牛磺酸(Tau)对实验性肝纤维大鼠肝组织的保护作用。[方法]将120只实验用大鼠随机分为正常对照组、模型组、牛磺酸低(150 mg/kg)、中(300 mg/kg)、高(600 mg/kg)剂量组和复方鳖甲软肝片540 mg/kg组,每组20只;采用CCl4复合法制备肝硬化门静脉高压大鼠模型,灌胃给药治疗4周后,测定血清中丙氨酸氨基转移酶门冬氨酸(ALT)、氨基转移酶(AST)和总胆红素(TBIL)含量,测定血清中肝纤四项(CⅣ、PCⅢ、LN、HA)含量,HE染色法观察肝脏组织形态结构变化并对肝组织细胞坏死和组织增生进行分级;通过天狼星染色观察肝脏组织纤维化状况;测定抗氧化酶(SOD、GSH-Px)活性和丙二醛(MDA)含量;测定血清中炎症细胞因子IL-10、TNF-α含量。[结果]与模型组相比,牛磺酸中、高剂量组大鼠血清中ALT、AST、TBIL含量和CⅣ、PCⅢ、HA含量均显著降低(P<0.05,P<0.01),高剂量组LN含量显著降低(P<0.05);牛磺酸各剂量组大鼠肝组织纤维化病变呈不同程度改善,以高剂量组效果最为显著;牛磺酸中、高剂量组大鼠肝脏组织SOD、GSH-Px活性显著升高且MDA含量显著降低(P<0.05,P<0.01),血清中IL-10、TNF-α含量显著降低。[结论]牛磺酸对实验性肝纤维化大鼠具有保护作用,其机制可能与牛磺酸能够有效保肝降酶、抑制氧化应激反应和炎症反应、改善肝功能有关。[Objective] To investigate the protective effects ofTaurine （Tau） on the experimental hepatic fibrosis rats. [Methods] The 120 experimental rats were randomly divided into six groups evenly： control group, model group, Tau group with low dosage （150 mg/kg）, Tau group with medium dosage （300mg/kg）, Tau group with high dosage （600 mg/kg） and Fufang Biejia Rangan Pian group （540 mg/kg）. The liver cirrhosis portal hypertension rat model rat models were made by injecting CCl4. After 4 weeks of giving the drugs by intragastric administration once a day, the content of ALT, AST, TBIL, CⅣ, PC Ⅲ, LN, HA in serum were determined. Observed the histopathological changes of the hepatic tissue by HE staining, and graded the liver tissue necrosis and tissue proliferate; observed the liver tissue fibration situation by sirius staining, determined the activity of SOD, GSH-Px and the content of MDA in hepatic tissue and the content of IL-10, TNF-α in serum. [Re sults] Compared with model control group, the content of ALT, AST, TBIL, CⅣ, PC Ⅲ, HA in serum and the HYP in hepatic tissue of Tau medium in high dosage groups were significantly decreased （P〈0.05, P〈0.01）, and the content of LN was significantly decreased （P〈0.05）; hepatic tissue histopath-ological changes ofTau treated groups were significantly improved, especially the high dosage group. The activity of SOD, GSH-Px in hepatic tissue of Tau group with medium, high dosage treated groups were significantly increased and the content of MDA were significantly decreased （P〈0.05, P〈0.01）; the content ofIL-10, TNF-α in serum were significantly decreased （P〈0.05, P〈0.01）. [Conclusion] Tau had protective effects to the hepatic tissue of experimental hepatic fibrosis rats, which may related with its effects of enzyme activity inhibition and hepatic tissue protection, inhibiting oxidative stress and inflammation and improving liver function.

关 键 词：牛磺酸 肝纤维化 大鼠 保护 

分 类 号：R285.5[医药卫生—中药学]