伪狂犬病毒法示踪成年大鼠排便反射神经通路的研究  

A study of defecation neural pathway of inoculating pseudorabies virus into rectum in adult rats

作　　者：王琦[1] 向波[1] 李亚楠[1] 赵一洋[1] 

机构地区：[1]四川大学华西医院小儿外科,四川省成都市610041

出　　处：《临床小儿外科杂志》2017年第2期185-188,共4页Journal of Clinical Pediatric Surgery

基　　金：四川省科技厅应用基础研究项目(2014JY0211)

摘　　要：目的通过伪狂犬病毒示踪技术,探索成年大鼠排便反射相关的神经通路。方法选择正常成年SD大鼠(体重230~250 g)14只,随机分为实验组和对照组,实验组(10只)于末端直肠壁内注射伪狂犬病毒(Bartha株)10~20μL;对照组A(2只)经肛门向直肠腔内注射伪狂犬病毒(Bartha株)20μL,对照组B(2只)于腹腔内注射伪狂犬病毒(Bartha株)20μL。两组大鼠均于注射病毒96 h后处死,取出脑干及脊髓,进行免疫组化染色分析。结果实验组大鼠的脊髓各节段中均出现伪狂犬病毒阳性的神经元细胞,其中T13~L1、L6~S1节段中病毒阳性的神经元数量较多(T13:47.5±2.9;L1:64.5±2.5;L6:30±2.1;S1:68.5±3.5),脑干Barrington氏神经核团处亦出现伪狂犬病毒阳性的神经元细胞(143.5±8.1)。对照组大鼠的脊髓及脑干内均未检测出伪狂犬病毒阳性细胞。结论实验组的伪狂犬病毒示踪结果具有较高的特异性;成年大鼠的排便反射神经通路在脊髓内与L6至S1以及T13至L1节段关系密切,在脑干内则与Barrington氏神经核团关系密切。Objective To explore the defecation neural pathway in spine and brainstem by inoculating pseudorabies virus （PRV, Bartha strain） into rectum in adult rats. Methods A total of 14 normal adult SD rats （male or female, weight： 230 -250 g） were randomly divided into two groups. The experimental group （n = 10） received an inoculation of 10 -20 μL PRY into rectal wall. The control group A had an injection of 20 μl PRV into peritoneal cavity （ n = 2 ） while the control group B 20 μL PRV into rectal cavity （ n = 2 ）. At 96 h post-injection, the rats were sacrificed for harvesting spine and brainstem for immunohistochemical staining and analysis. Results PRV-immunoreactive （IR） cells were localized into almost cvery spinal cord in experi- mental rats, especially spinal segments of T13 - L1 and L6 - S1 （T13 ： 47.5 ±2.9 ; L1 ： 64.5 ± 2.5 ; L6 ： 30 ± 2. l; S1：68.5 ±3.5）. And PRV-IR cells were also detected in Barrington＇ s nucleus （143.5± 8.1 ） in brainstem of experimental rats. And PRV-IR cells were not detected in control rats. Conclusions PRV trans- neuronal tracing has a high specificity in experimental rats. And the defecation neural pathway is closely associ- ated with spinal segments of T13-L1 and L6-S1 and Barrington＇ s nucleus in adult rats.

关 键 词：狂犬病病毒 大鼠 排便 反射 神经通路 脊髓 

分 类 号：R338[医药卫生—人体生理学]