肝癌患者腹腔积液中可溶性程序性死亡蛋白1(sPD-1)及其配体表达水平  

Expressions of soluble programmed death 1 and its ligands in ascites in patients with hepatocellular carcinoma

作　　者：刘伟[1] 杨记康 张增增[1] 鲁之中[1] 杨思伟[1] 

机构地区：[1]焦作市人民医院检验科,河南焦作454002

出　　处：《微生物学免疫学进展》2017年第2期24-28,共5页Progress In Microbiology and Immunology

基　　金：焦作市科技攻关项目(2014130005)

摘　　要：目的观察肝细胞肝癌(hepatocellular carcinoma,HCC)患者腹腔积液中可溶性程序性死亡蛋白1(solubleprogrammed death-1,s PD-1)及其配体水平。方法实验分为早期HCC组(20例)、进展期HCC组(15例)及乙型肝炎肝硬化患者对照组(15例),分别采集3组人群的腹腔积液,利用RT-PCR扩增腹腔积液细胞中程序性死亡蛋白1(programmed death 1,PD-1)及其配体PD-L1基因,用ELISA检测腹腔积液上清中s PD-1及其配体s PD-L1和IFN-γ水平,并分析s PD-1、s PD-L1与IFN-γ的相关性。结果早期HCC组和进展期HCC组的PD-1、PD-L1基因表达均高于对照组;进展期HCC组腹腔积液中s PD-1、s PD-L1和IFN-γ均高于对照组和早期HCC组,差异均具有统计学意义(P<0.05);且s PD-1、s PD-L1与IFN-γ呈正相关。结论 s PD-1、s PD-L1在HCC患者腹腔积液中高表达,有望成为HCC免疫治疗的新靶点。Objective To observe the levels of soluble programmed death protein 1 （ sPD-1 ） and its ligands in ascites of patients with hepatocellular carcinoma（HCC）. Methods The experiement was conducted in 3 groups including 20 patients with early HCC, 15 patients with progressive HCC and a cotrol group of 15 patients with hepatocirrhosis of hepatitis B, respectively, and ascites were collected from the subjects. The expressions of PD-I and PD-L1 in ascites cells were amplified and detected by RT-PCR, the levels of sPD-1, sPD-L1 and IFN-γ in the supernatant from ascites were determined by ELISA. The correlation of sPD-1, sPD-L1 with IFN-γ was analyzed with Pearson＇s correlation and One-way ANOVA. Results The expression levels of PD-1 and PD-L1 in the early HCC and the progressive HCC groups were both higher than those in the control group, sPD-1 and sPD-L1 in progressive HCC group were higher compared with the control group and early HCC group, there was a statistically meaning （P〈0.05） , in addition, the expression levels of sPD-land sPD-L1 were positively correlated with expression of IFN-γ. Conclusion The high expression levels of sPD-1 and sPD-LI in the ascites of HCC patients are likely to become the new targets for HCC immunotherapy.

关 键 词：肝细胞肝癌 腹腔积液 可溶性程序性死亡蛋白1 可溶性程序性死亡蛋白配体1 Γ干扰素 

分 类 号：R735.7[医药卫生—肿瘤]