结膜松弛症手术切除的结膜中细胞因子的表达  

Expression of cytokines in the conjunctiva excised from conjunctivochalasis

作　　者：项敏泓[1] 王晗敏[1] 贾元玲 周欢明[1] 陈星星[1] 张兴儒[1] 

机构地区：[1]上海中医药大学附属普陀医院眼科,上海200062

出　　处：《中华眼外伤职业眼病杂志》2017年第4期241-244,共4页Chinese Journal of Ocular Trauma and Occupational Eye Disease

基　　金：上海市卫生及计划生育委员会资助项目（20124108）;上海中医药大学预算内项目（2015YSNS0）

摘　　要：目的 观察结膜松弛症手术切除的松弛结膜组织中肿瘤坏死因子-α（TNF-α）及白介素-1β（IL-1β）的表达情况.方法 结膜松弛症组15例（15只眼）,并选年龄匹配的翼状胬肉组10例（10只眼）和正常对照组9例（9只眼）.对3个组切除的结膜组织行TNF-α和IL-1β的免疫荧光染色观察,并作统计学分析.结果 结膜组织中TNF-α阳性表达面积：结膜松弛症组为（712.44±33.84） μm^2,翼状胬肉组为（650.14±31.76） μm^2,正常对照组为（633.25±15.88） μm^2,差异具有统计学意义（F=12.729,P=0.000）.结膜组织中IL-1β阳性表达面积：结膜松弛症组为（722.11±60.09） μm^2,翼状胬肉组为（664.71±33.10）μm^2,正常对照组为（627.75±48.83）μm^2,差异具有统计学意义（F=5.648,P=0.013）.结论 结膜松弛症的松弛结膜组织中TNF-α、IL-1β表达增强,进一步证实结膜松弛症与炎症相关.Objective To observe the expression of tumor necrosis factor-α（TNF-α）and interleukin-1β（IL-1β） of surgical excised conjunctival tissue from the conjunctivochalasis patients.Methods Thirty-four patients were divided into 3 groups： conjunctivochalasis group in 15 eyes of 15 cases, age matched patients with pterygium group in 10 eyes of 10 cases and normalcontrol group in 9 eyes of 9 cases.The excised conjunctival tissues of 3 groups were stained with TNF-α and IL-1β immunofluorescence staining.The results were observed and statistically analyzed.Results The positive cells area of TNF-α in conjunctival tissue from the conjunctivochalasis group was （712.44±33.84） μm^2, （650.14±31.76） μm^2 from the pterygium group, and （633.25±15.88） μm^2 from normal control group,the difference of TNF-α among 3 groups were statistically significant （F=12.729,P=0.000）.The positive cells area of IL-1β in conjunctival tissue from the conjunctivochalasis group was （722.11±60.09） μm^2, （664.71±33.10） μm^2 from the pterygium group, and （627.75±48.83） μm^2 from the normal control group, the difference among 3 groups were statistically significant （F=5.648,P=0.013）.Conclusion The expression of TNF-α and IL-1β in loose conjunctival tissue from conjunctivochalasis is enhanced, which further confirms that inflammation is associated with conjunctivochalasis.

关 键 词：结膜松弛症 结膜组织 细胞因子 肿瘤坏死因子-Α 白介素-1Β 

分 类 号：R779.6[医药卫生—眼科]