不同根管消毒方法对粪肠球菌清除作用的体外研究  

Elimination of Entercoccus faecalis with different disinfection methods in root canals in vitro

作　　者：孙楚文 朱亚琴[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院.口腔医学院口腔综合科上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2017年第2期134-138,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81271134);上海高校高峰高原学科建设项目

摘　　要：目的 :比较被动超声荡洗(passive ultrasonic irrigation,PUI)与半导体激光,及两者联用对根管内粪肠球菌的清除作用,并与3%Na Cl O消毒效果进行比较,为临床选择根管消毒方法提供参考。方法:选取50颗成人单根管离体前磨牙,建立粪肠球菌生物膜感染根管模型,随机将其均分为5组并做不同处理。A组为生理盐水冲洗组,B组为3%Na Cl O溶液冲洗组(阳性对照组),C组为PUI处理组,D组为半导体激光处理组,E组为PUI联合半导体激光处理组。选用纸尖法取样,稀释培养48 h后,计算每个样本菌落形成单位(colony-forming units,CFU)值。采用SAS 8.02软件包对数据进行统计学分析。结果:PUI处理组、半导体激光处理组及联合处理组样本的CFU值,与生理盐水冲洗组相比均存在显著差异(P<0.01),3组结果均显著低于生理盐水冲洗组,而3组之间则无显著差异(P>0.05)。3%Na Cl O溶液冲洗组(阳性对照组)的CFU值均显著低于其余4组(P<0.01)。结论:PUI、半导体激光消毒及其两者联用均对粪肠球菌生物膜具有显著消毒效果,显著优于单纯使用生理盐水冲洗,但三者之间效果相当;3%Na Cl O冲洗对粪肠球菌生物膜具有最强的消毒作用。PURPOSE： To compare the elimination effect against E.faecalis in root canals with different methods. METHODS： Fifty extracted premolars with single root canal were selected. After cleaning and autoclaving, they were contaminated by E.faecalis and incubated for 28 days as models. Then the models were randomly and equally divided into 5 groups and treated as below： specimens in group A were treated with saline irrigation, specimens in group B were treated with 3% NaCIO irrigation （as positive control）, specimens in group C were treated with PUI, specimens in group D were treated with diode laser radiation, specimens in group E were treated with combination of PUI and diode laser radiation. The specimens from root canals were collected by paper points. The bacterial suspensions were later serially diluted and plated on tryptic soy agar plates to enumerate the CFUs after 48 hours. Statistical analysis was performed using SAS 8.02 software package. RESULTS： As with all parts of the root canal in aggregate, the CFUs of the specimens treated with PUI, diode laser radiation and the combination of them were significantly lower than the specimens treated with saline irrigation （P〈0.01）, but there was no significant difference among 3 groups （P〉0.05）. However, the specimens treated with 3% NaCIO irrigation had the best effect of disinfection. The number of CFUs in the specimens treated with 3% NaC10 was almost zero. There was no significant difference between this group and others in CFUs （P〈0.01）. CONCLUSIONS： Specimens treated with PUI, diode laser radiation and the combination of them showed great effect of elimination against biofilm of Enterococcus faecaIis compared with saline irrigation. Irrigation with 3% NaC10 was the most efficient method in this experiment.

关 键 词：根管消毒 粪肠球菌 次氯酸钠 被动超声荡洗 半导体激光 

分 类 号：R781.05[医药卫生—口腔医学]