不同溶剂柠檬果皮提取物抗氧化、抑菌活性比较及其与多酚组成的关系  

Comparsion of Antioxidant and Antifungal Activities of Various Solvent Extracts of Lemon Peel and Analysis the Relationship with Polyphenol Composition

作　　者：左龙亚 滕左 王孝仕 江东[3] 于杰[1,4] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]重庆市梁平县农业委员会,重庆405200 [3]西南大学柑桔研究所,重庆400712 [4]南方山地园艺学教育部重点实验室,重庆400715

出　　处：《园艺学报》2017年第4期743-754,共12页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31171930);重庆市自然科学基金项目(CSTC2013JCYJA8002);中央高校基本科研业务费专项资金项目(XDJK2014C091);重庆北碚区科技计划项目(2015-35)

摘　　要：以柠檬果皮为材料,探讨不同溶剂提取对粗提物抗氧化及抑菌活性的影响,并分析了各提取物多酚组成与抗氧化能力及抑菌活性的关系。结果表明,不同溶剂柠檬果皮提取物抗氧化能力及抑菌活性存在显著差异(P<0.05),抗氧化能力依次为:甲醇提取物>丙酮提取物>蒸馏水提取物>乙醇提取物>乙酸乙酯提取物,抑菌活性依次为:甲醇提取物>丙酮提取物>乙醇提取物>蒸馏水提取物>乙酸乙酯提取物。从柠檬果皮中共鉴定出16种多酚类物质,包括4种酚酸、12种类黄酮。相关性分析表明,不同提取物的多酚物质组成及其含量与抗氧化、抑菌活性密切相关,芸香柚皮苷、橙皮苷、新橙皮苷、没食子酸、绿原酸和咖啡酸对抗氧化活性的贡献较大,芸香柚皮苷、新橙皮苷、咖啡酸对抗菌活性的贡献最大。甲醇为最佳提取溶剂。The peel extracts of Citrus limon（L.）Burm. F. were obtained via an ultrasonic extraction method but with different solvents（methanol,ethanol,acetone,ethyl acetate,distilled water）. Their antioxidant and anti-fungi（Panicum italicum,Penicillium digitatum,Colletotrichum gloeosporioides）activities were tested. Further on the bioactive compounds of phenols from the extraction were analyzed by High Performance Liquid Chromatography（HPLC）. This study provides evidences for the comprehensive utilization of peels of Citrus limon（L.）Burm. F. Our studies suggested that the varieties and concentrations of phenolic compounds extracted from peels of Citrus limon（L.）Burm. F. via different solvents were with significant differences（P 〈 0.05）to antioxidant and anti-fungi activities. The solvents’ contributions to antioxidant and anti-fungi were listed in their descending order,methanol,acetone,distilled water,ethanol,ethyl acetate;methanol,acetone,ethanol,distilled water,ethyl acetate. There were 16 phenolic bioactive compounds can be detected with the extraction method with different solvents,including 4 phenolic acids and 12 flavonoids,their content were closely related to antioxidant and anti-fungi abilities. In terms of the bioactive compounds contributing to antioxidant abilities, narirutin, hesperidin,neohesperidin,galic acid,chlorogenic acid and caffeic acid were considered to play the most important roles;whereas contributing to anti-fungi abilities,narirutin,neohesperidin and caffeic acid were the best candidates. Methanol as the best solvent for peel extraction of Citrus limon（L.）Burm. F. can be used for promoting the antioxidant and anti-fungi activities.

关 键 词：柠檬 提取物 总酚 总黄酮 抗氧化抑菌性 高效液相色谱法 

分 类 号：S666[农业科学—果树学]