教学实验用阿米巴原虫培养方法的筛选与评价  

Screening and Evaluation of Amoeba Cultivation for Teaching and Experiment

作　　者：杨细凤[1] 李仲娟[1] 余福恒 李茉[1] 

机构地区：[1]湖北理工学院医学院,湖北黄石435003 [2]黄石市东方社区服务中心,湖北黄石435000

出　　处：《湖北理工学院学报》2017年第2期59-62,共4页Journal of Hubei Polytechnic University

摘　　要：为满足医学寄生虫课原虫教学实验所需,筛选来源于自然界的阿米巴原虫进行培养,以便找到更有效、便捷、经济且能满足临床教学所需阿米巴原虫的培养方法。采用1640细胞培养液、洛氏培养液、青菜水培养液培养来源于自然界的阿米巴原虫,观察其生长状态,分别比对阿米巴原虫到达包囊期和滋养体期所需的培养时间及不同时间复苏时的成活率。结果为:青菜水培养液方法对自然界的阿米巴原虫培养效果最佳,青菜水和1640培养液到达包囊阶段时间分别为(16±0.35)h、(18±0.50)h,与洛氏培养液到达包囊时间(22±1.2)h相比均有显著性差别(P<0.05);青菜水和1640培养液到达滋养体的时间分别为(22±2.24)h、(26±3.45)h,与洛氏培养液到达滋养体时间(30±2.5)h相比均有显著性差别(P<0.01);青菜水培养液和1640培养液6个月后再次复苏成活率分别为88%、78%,与洛氏培养液的62%相比均有显著性差别(P<0.01)。青菜水培养液法可作为自然界的阿米巴原虫培养的首选方法,培养原虫时间较短、复苏成活率高,且经济安全,培养的原虫可作为人体寄生虫学实践教学用样本的替代品。Objective： To establish a sound, easy and economic culturing system for the cultivation of amoeba protozoa from natural sources in order to meet the need for protozoa experimental teaching of hospitals. Methods ：Amoeba protozoa were cultured with 1640 cell culture media, Rockwell culture medium and vegetables water culture medium respectively. Growth status, development times of cysts and trophozoites and the survival rate at different recovering time were compared among the 3 groups. Results：The cysts stage time for amoeba protozoa culture with vegetables water culture medium and 1640 cell culture medium were （ 16±0.35 ） h and （ 18±0.50） h which had significant difference （ P 〈 0.05 ） from that with Rockwell culture medium, （ 22±1.2） h. The nourish body stage time for amoeba protozoa culture with vegetables water culture medium and 1640 cell culture medium were （ 22±2.24 ） h and （ 26±3.45 ） h, which also had extremely significant difference（ P 〈0.01 ） from that with Rockwell culture medium（30 ±2.5 ） h. Recovering survival rate for a- moeba protozoa culture with vegetables water culture medium and 1 640 cell culture medium after 6 months were as high as 88% and 78% , which had extremely significant difference（ P 〈0.01 ） from that with Rock-well culture medium ,62%. Conclusion ：The culture time of amoeba protozoa from natural sources with vegeta- bles water culture medium is short and its recovering survival rate is high. So, vegetables water culture medi- um can be used as a preferred medium for the culture of amoeba protozoa from natural sources and can be a substitute for the test sample of Human Parasitology teaching practice.

关 键 词：教学实验 原虫 培养液 阿米巴原虫 

分 类 号：R382.1[医药卫生—医学寄生虫学]