Study on Efficient in Vitro Embryo Production Technology of Lambs  

羔羊体外胚胎高效生产技术研究（英文）

作　　者：佟桂芝[1] 李信涛 宋斌[1] 董正德[1] 王伟霞 海龙[1] 刘小辉 郭立宏[1] 王嘉厚[1] 王植朔[1] 

机构地区：[1]黑龙江省畜牧研究所,黑龙江齐齐哈尔161005 [2]吉林省农业科学研究院,吉林长春130000

出　　处：《Agricultural Science & Technology》2017年第4期678-679,686,共3页农业科学与技术（英文版）

基　　金：Supported by the Project of the Finance Department of Heilongjiang Province(2016-10);the Key Program for Agriculture of Qiqihar City(NYGG-201425)~~

摘　　要：The aim was to compare the effects of hormone treatments on the ovari- an follicular development and in vitro embryo development between 5-week and 13- week old lambs. FSH and pregnant mare serum gonadotropin （PMSG） treatment were used to compare the number of 2-8 mm follicles in the ovary and the in vitro embryo development. The results showed that： the 5-week old lambs had larger fol- licular numbers and collected oocytes numbers （58.6±1.9, 47.4±4.2） than the 13- week old lambs （14.4±4.1, 11.6±1.7）, and the difference was significant （P〈0.05）. However, there was no significant difference in the cleavage rate （60.0±1.9%, 61.6± 5.2%） and blastocyst rate （27.4±2.1%, 28.2±3.7%） of the fertilized oocytes between the 5-week and 13-week old lambs.该研究旨在比较良种肉羊5周龄、13周龄羔羊激素处理后卵巢卵泡发育及体外胚胎发育的影响。通过FSH和孕马血清促性腺激素(PMSG)处理,比较卵巢上2~8 mm卵泡的数量及体外胚胎的发育情况。结果表明:5周龄羔羊卵巢发育的卵泡数和采集到的卵母细胞数(58.6±1.9、47.4±4.2)都高于13周龄羔羊卵泡数和卵母细胞数(14.4±4.1、11.6±1.7),且差异显著(P<0.05)。5、13周龄羔羊卵母细胞受精后的卵裂率(60.0%±1.9%、61.6%±5.2%)及囊胚率(27.4%±2.1%、28.2%±3.7%),两者间差异不显著。

关 键 词：HORMONE Month age Follicle LAMB In vitro embryo 

分 类 号：S826[农业科学—畜牧学]