纳米炭吸附表阿霉素对人乳腺癌MCF-7细胞增殖及凋亡的影响  

Effects of carbon nanoparticles-epirubicin suspension on cell proliferation and apoptosis in breast cancer MCF-7 cells

作　　者：张永松[1] 梁全琨 钟玲[1] 齐晓伟[1] 宗贝歌[1] 杜俊泽 张毅[1] 范林军[1] 

机构地区：[1]重庆第三军医大学西南医院乳腺中心,400038

出　　处：《中华内分泌外科杂志》2017年第2期110-114,共5页Chinese Journal of Endocrine Surgery

基　　金：重庆市科技攻关项目（CSTC2011AB05041）

摘　　要：目的探讨纳米炭吸附表阿霉素（carbon nanoparticles-epirubicin suspension，CNP-EPI）在体外对人乳腺癌MCF-7细胞增殖和凋亡的影响。方法采用不同浓度的CNP-EPI处理MCF-7细胞，CCK-8法测定不同浓度、不同时间对MCF-7细胞增殖的抑制作用；根据测定的IC50选取5 μg/ml CNP-EPI作用于细胞24 h后，观察细胞形态的变化情况，流式细胞仪（flow cytometry，FCM）测定细胞凋亡率。结果在1~200 μg/ml浓度范围内，分别在24 h、48 h、72 h时间点内，CNP-EPI对乳腺癌MCF-7细胞均有增殖抑制的作用，与正常对照组比较，差异有统计学意义（P〈0.01），且各时间段内对细胞的抑制率与药物浓度呈剂量依赖关系，随药物浓度的增加抑制作用逐渐增强，相同浓度药物随时间的延长对细胞的抑制作用有所减弱,但72 h对细胞仍有较强的抑制作用，而相应浓度纳米炭不同时间对MCF-7细胞的增殖无明显抑制作用；5 μg/ml CNP-EPI作用MCF-7细胞24 h后，显微镜下可看到大量细胞死亡，正常对照组和相应浓度的纳米炭组细胞未见明显异常，流式细胞仪检测，CNP-EPI组作用细胞24 h后，晚期凋亡率为（14.57±2.41）%，与相应浓度纳米炭组和正常对照组比较，差异有统计学意义（P〈0.05），且CNP-EPI组对细胞的死亡率可达（78.63±0.55）%。结论CNP-EPI混悬液对人乳腺癌MCF-7细胞增殖有明显抑制或杀伤作用，其抑制作用可能是通过其缓慢释放的表阿霉素直接杀伤癌细胞或诱导其凋亡实现。ObjectiveTo explore the effect of carbon nanoparticles-epirubicin suspension on proliferation and apoptosis in human breast cancer MCF-7 cells.MethodsMCF-7 cells were cultured with different concentrations of CNP-EPI in vitro. CCK-8 assay was used for determinate inhibition effect of CNP-EPI on the proliferation of MCF-7 cells at different concentration and different time. According to the determination of IC50, 5 μg/ml CNP-EPI was selected, and cell morphology and cell apoptosis rates were observed after 24 h.ResultsThe inhibition effect of the CNP-EPI was stronger significantly in CNP-EPI group than in normal control group within 24 h, 48 h, 72 h when the concentration was from 1 μg/ml to 200 μg/ml （P〈0.01） .The inhibition of CNP-EPI on the proliferation of MCF-7 cells was gradually strengthened in a dose-dependent relation within the same time, and the inhibition effect is reduced in the same concentration of drugs with the time extension, but it still has a strong inhibitory effect in 72 h, and the inhibition effect of different concentration of CNP was not obvious on MCF-7 cells. Obvious changes of cell morphology were observed under inverted microscope such as： a lot of dead cells, cell adherent growth poor, cell morphology became round and karyopycnosis etc, in 5 μg/ml CNP-EPI group after 24 h. However, no obvious abnormity of cell morphology was observed in normal control group and corresponding CNP group. Late apoptosis rate was （14.57±2.41） %, the mortality rate could reach （78.63±0.55） % in 5 μg/ml CNP-EPI group after 24 h. The mortality rate and apoptosis rate of cells was higher significantly in CNP-EPI group than in CNP group and normal control group （P〈0.05） .ConclusionCNP-EPI can obviously inhibit the proliferation or kill human breast cancer MCF-7cells, and the inhibition effect of CNP-EPI on proliferation of breast cancer cells might be the result of delayed releasing of EPI.

关 键 词：纳米炭 表阿霉素 MCF-7细胞 增殖 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]