杜仲不同部位总黄酮含量及抗氧化活性研究  

Study on the Total Flavonoids Content and Antioxidant Activity in Different Parts of Eucommiae ulmoides

作　　者：钟淑娟[1] 杨欣[1] 李静[2] 李咏梅[1] 黎行山[1] 

机构地区：[1]广州医科大学附属第五医院药学部,广州510700 [2]广州中医药大学中药学院,广州510006

出　　处：《中国药房》2017年第13期1787-1790,共4页China Pharmacy

摘　　要：目的:比较杜仲皮、叶、雄花及籽中总黄酮含量与抗氧化活性。方法:采用紫外分光光度法测定各部位总黄酮含量;以半数清除/还原浓度(IC_(50))值为评价指标,对各部位样品进行清除2,2′-联氮基双(3-乙基苯并噻唑啉-6-磺酸)(ABTS^+)、1,1-二苯基-2-三硝基苯肼(DPPH)自由基试验以及对铜离子(Cu^(2+))的还原能力试验,并以维生素C为阳性对照。结果:杜仲不同部位总黄酮含量由高到低为叶>雄花>皮>籽,其中除杜仲皮与籽总黄酮含量比较差异无统计学意义外(P>0.05),其余各部位间比较差异均有统计学意义(P<0.05);各样品对ATBS^+、DPPH自由基清除作用由强到弱为叶>雄花>籽>皮,其中除杜仲叶与雄花比较各指标差异无统计学意义外(P>0.05),其余各部位间比较差异均有统计学意义(P<0.05);各样品对Cu^(2+)还原能力由强到弱为叶>雄花>皮>籽,其中叶、雄花与皮、籽比较差异有统计学意义(P<0.05)。结论:杜仲叶与雄花部位总黄酮含量较高、抗氧化活性较强,可进行相应的开发利用以弥补传统药用部位杜仲皮资源的不足。OBJECTIVE：To compare the total flavonoids content and antioxidant activity in the barks,leaves,male flowers and seeds of Eucommiae ulmoides. METHODS：UV spectrophotometry was used to determine the total flavonoids content in differ-ent parts;tests was conducted to clear 2,2′-nitrilobis（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS＋）,1,1-diphenyl-2-trinitro-phenylhydrazine（DPPH）radicals and the reducing ability of Cu2＋,using half clear/reduction concentration value（IC50）as evalua-tion indexes,and vitamin C was regarded as positive control. RESULTS：The total flavonoids content of the E. ulmoides from hight to low was as follows as leaves〉male flowers〉barks〉seeds,except there was no significant difference in barks and seeds （P〉0.05）,the other parts had significant differences （P〈0.05）;the ability of different parts eliminating DPPH and ABTS＋ free radical was as follows as leaves〉male flowers〉seeds〉barks,except there was no significant difference in the indicators of leaves and male flowers（P〉0.05）,the other parts had significant differences（P〈0.05）;the ability of reducing Cu2＋ free radical was as follows as leaves〉male flowers〉barks〉seeds,there was significant difference in leaves and males flowers with barks and seeds （P〈0.05）. CONCLUSIONS：The content of total flavonoids in leaves and male flowers is high,and the antioxidant activity is strong,which has a great prospect of exploitation and utilization to make up for deficiencies in barks of E. ulmoides.

关 键 词：杜仲 皮 叶 雄花 籽 总黄酮 抗氧化活性 

分 类 号：R285.5[医药卫生—中药学]