超声波-酶法提取积雪草中积雪草总苷的工艺研究  

Study on the Extraction Technology of Asiaticosides from Centella asiatica by Ultrasonic-enzyme Method

作　　者：张丹丹[1] 聂绪强[1] 张涵[1] 赵久凤[1] 石秀君[1] 杨建文[1,2] 

机构地区：[1]遵义医学院药学院,贵州遵义563003 [2]遵义医学院附属医院,贵州遵义563003

出　　处：《中国药房》2017年第13期1816-1819,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81560712);贵州省科学技术基金项目(No.黔科合LH字[2014]7566号);贵州省科技计划课题(No.黔科合重大专项字[2015]6010)

摘　　要：目的:优化积雪草中积雪草总苷的提取工艺。方法:以积雪草苷和羟基积雪草苷含量之和为考察指标,采用单因素试验和正交试验对酶用量、酶解时间、酶解温度、乙醇体积分数、液料比、超声提取时间进行考察,优化超声波-酶法提取积雪草中积雪草总苷的工艺条件并进行验证试验。结果:最优提取工艺为纤维素酶用量12 mg/g,以10倍液料比加入60%乙醇,60℃酶解60min,超声提取50 min;验证试验中积雪草总苷平均提取率为1.92%(RSD=1.83%,n=3)。结论:超声波-酶法用于积雪草中积雪草总苷的提取,工艺稳定、可行。OBJECTIVE：To optimize the extraction technology of asiaticosides from Centella asiatica. METHODS：Using to-tal amounts of asiaticoside and hydroxy asiaticoside as investigation indexes,single factor and orthogonal test were used to investi-gate the enzyme amount,enzymolysis time,enzymolysis temperature,ethanol volume fraction,liquid material ratio and ultrasonic extraction time and optimize the extraction technology of asiaticosides from C. asiatica by ultrasonic-enzyme method,and verifica-tion test was conducted. RESULTS：Optimal extraction technology was as follow as cellulose dosage of 12 mg/g,10-fold liquid ma-terial ratio added into 60% ethanol,enzyme hydrolysis for 60 min at 60 ℃,ultrasonic assisted extraction for 50 min. Average ex-traction rate of total asiaticosides was 1.92%（RSD=1.83%,n=3）in verification test. CONCLUSIONS：Ultrasonic-enzyme meth-od is stable and feasible for the extraction technology of asiaticosides from C. asiatica.

关 键 词：积雪草 积雪草苷 羟基积雪草苷 超声波 纤维素酶 提取工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学]