多指标正交试验优化复方红景天颗粒的提取工艺  

Optimization of Extraction Technology of Compound Rhodiolae crenulatae Granule by Multi-index Orthogonal Test

作　　者：刘庆焕[1] 王宏[1] 张婷[1] 张岩[1] 王文彤[1] 陶遵威[1] 

机构地区：[1]天津市医药科学研究所,天津300020

出　　处：《中国药房》2017年第13期1830-1833,共4页China Pharmacy

基　　金：天津市应用基础与前沿技术研究计划资助项目(No.14JCYBJC28700)

摘　　要：目的:优化复方红景天颗粒的药材提取工艺。方法:以药效学指标(学习记忆能力)对小鼠进行Y型水迷宫实验,筛选复方红景天颗粒药材的水提、水提醇沉及醇提方法;以红景天苷、阿魏酸提取量及出膏率的综合评分为指标,采用正交试验优化醇提工艺中乙醇体积分数、提取时间、溶剂用量和提取次数,并进行验证试验。结果:3种提取方法中以醇提方法所得产物对改善学习记忆能力效果最好;最优醇提工艺为8倍量70%乙醇回流提取3次,每次1.0 h。验证试验中红景天苷、阿魏酸的提取量及出膏率平均值分别为4.884 mg/g、1.669 mg/g、33.52%(RSD均小于2%,n=3)。结论:优化的提取工艺稳定、可靠、简便、易行,可用于复方红景天颗粒的工业化提取。OBJECTIVE：To optimize the extraction technology of Compound Rhodiolae crenulatae granule. METHODS：The pharmacodynamic index of learning and memory ability was used to screen the water extraction,water extraction and alcohol pre-cipitation and alcohol extraction methods for Compound R. crenulatae granule in mice by Y-type water maze test. Using the compre-hensive scores of salidroside,ferulic acid extraction amount and extraction yield as indexes,orthogonal test was used to optimize the ethanol volume fraction,extraction time,solvent amount and extraction times,and verification test was conducted. RESULTS：Products by alcohol extraction can significantly improve learning and memory ability in the 3 methods;the optimal extraction tech-nology was confirmed to 8-fold 70% ethanol reflux extracting for 3 times,1.0 h each time. In verification test,average extraction amount of salidroside and ferulic acid were 4.884 mg/g and 1.669 mg/g,and average extraction yield was 33.52%（all RSD〈2%, n=3）. CONCLUSIONS：The optimized extraction technology is stable,reliable,simple and feasible,which can be used for indus-trial extraction of Compound R. crenulatae granule.

关 键 词：复方红景天颗粒 提取工艺 药效指标 小鼠 红景天苷 阿魏酸 正交试验 

分 类 号：R284.2[医药卫生—中药学]