核心岩藻糖基转移酶Fut8基因敲除小鼠肠道菌群特征  

Intestinal microbial structure of core fucosyltransferase Fut8 gene knockout mice

作　　者：李明[1] 白亚强[1] 何春阳[1,2] 鄢秋龙[1] 房慧[1] 李志[3] 袁杰力[1] 李文哲[1] 

机构地区：[1]大连医科大学基础医学院,辽宁大连116044 [2]哈尔滨商业大学药学院,黑龙江哈尔滨150000 [3]大连市中心医院检验科,辽宁大连116044

出　　处：《中国微生态学杂志》2017年第4期381-384,389,共5页Chinese Journal of Microecology

基　　金：国家自然科学基金面上项目(31570797/31270864);辽宁省自然科学基金(2015020253/2015010262);辽宁省教育厅科学研究项目(L2016003)

摘　　要：目的探究Fut8基因敲除对ICR小鼠肠道菌群结构的影响。方法分别在Fut8^(+/+)和Fut8^(-/-)小鼠出生后不同时间点检测体重,采集粪便样本,采用PCR-变性梯度凝胶电泳(PCR-DGGE)的方法检测小鼠粪便中菌群的组成;利用高效液相色谱法(HPLC)测定Fut8^(+/+)和Fut8^(-/-)小鼠Fut8酶活。结果 Fut8^(-/-)小鼠与Fut8^(+/+)小鼠相比生长缓慢(P<0.05);完全丧失核心岩藻糖基化活性;肠道菌群结构不同,其中拟杆菌门微生物存在显著差异。结论核心岩藻糖基转移酶Fut8基因敲除对小鼠肠道菌群结构有显著影响。Objective To determine the effect of Fur8 gene knockout on the structure of intestinal microbiota in ICR mice. Methods The body weights were measured at different time points after Fur8＋/＋ and Fut8 / mice were born. Fecal samples were collected at the same time to detect the intestinal microbiota by using PCR-Denaturing Gradient Gel Electrophoresis （PCR-DGGE） . The enzyme activity of Fut8 was assayed by using High Performance Liquid Chromatography （HPLC） . Results The Fur8-/- mice grew slowly com- pared with Fut8＋/＋ mice （P^0.05）, and the fucosyltransferase activity was abolished. Compared with the controls, the intestinal microbiota of Fut8-/ mice showed a different pattern, among which the bacteria that belong to the phylum of Bacteroidetes changed significantly. Conclusion Core fucosyltransferase （FurS） gene knockout had a significant effect on the structure of intestinal microbiota in mice.

关 键 词：核心岩藻糖基转移酶 Fut8基因 基因敲除 肠道菌群 核心岩藻糖 

分 类 号：R378[医药卫生—病原生物学]