重组杆状病毒介导的DNMT1 siRNA对人胰腺癌细胞增殖影响的研究  

Effects of DNMT1 siRNA Recombinant Baculovirus on Pancreatic Cancer Cell Proliferation

作　　者：郭柔玉[1] 曹亮[1] 

机构地区：[1]江苏大学附属医院消化内科,江苏省镇江市212001

出　　处：《医学理论与实践》2017年第8期1100-1101,共2页The Journal of Medical Theory and Practice

摘　　要：目的:研究利用重组杆状病毒介导的DNMT1siRNA对人胰腺癌细胞增殖的影响。方法:培养人胰腺癌细胞并分组如下:空白对照组、阴性对照组、病毒r-Bac-si-DNMT1-D1组(D1组)、r-Bac-si-DNMT1-D2组(D2组)、r-Bac-siDNMT1-D3组(D3组)。空白对照组仅加10%胎牛血清和DMEM培养基培养,阴性对照组、D1组、D2组、D3组加血清和DMEM培养基培养24h后依次加入空白载体r-Bac-CMV-EGFP和r-Bac-si-DNMT1-D1病毒、r-Bac-si-DNMT1-D2病毒、r-Bac-si-DNMT1-D3病毒150μl/孔,72h后分别收集各组细胞用MTT法测肿瘤细胞增殖的变化。结果:重组杆状病毒介导的DNMT1siRNA感染的细胞存活率较空白对照组和阴性对照组明显降低(P<0.05)。结论:杆状病毒介导的DNMT1siRNA感染人胰腺癌细胞后能通过降低细胞存活率,从而影响胰腺癌细胞的增殖。Objective：This study is to observe the effects of DNMT1 siRNA recombinant baculovirus on pancreatic cancer cell. Methods：Cultured Human pancreatic cancer cell and divided into five groups： control group, negative control group, r-Bac-si-DNMT1-D1 group （D1 group）, r-Bac-si-DNMT1-D2 group （D2 group）, r-Bac-si-DNMT1-D3 group （D3 group）. Control group were cultured only with 10% FBS and DMEM. At 24h after plating with FBS and DMEM, Negative control group, D1 group, D2 group,D3 group were transfected with corresponding baculovirus-mediated RNA interference of DNMTl（150μl/well）. And after 72h transfection, use MTT to test the change of cell proliferation. Results： The cell survival rate of DNMT1 siRNA recombinant baculovirus group were significant lower than that in control group and negative control group （P^0. 05）. Conclusion： DNMTI siRNA recombinant baculovirus could deduce proliferation on human pancreatic cancer by lower the cell survival rate.

关 键 词：重组杆状病毒 DNMT1 SIRNA 胰腺癌 增殖 

分 类 号：R735.9[医药卫生—肿瘤]