微小RNA-31在乳腺癌细胞中低表达对乳腺癌细胞侵袭的影响  

Effect of down - regulation of microRNA - 31 on invasion of breast cancer cells

作　　者：柴丽君[1] 陈建中[1] 翟媛媛[1] 谢勇[2] 

机构地区：[1]郑州大学第二附属医院乳腺外科,450014 [2]北京大学深圳医院肝胆外科,深圳518032

出　　处：《中华实验外科杂志》2017年第4期562-564,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察微小RNA（miRNA，miR）-31在乳腺癌细胞中的表达以及对乳腺癌细胞的作用。 方法分子克隆构建重组pMSC-异源三聚体G蛋白13（GNA13） wt质粒，分别转染不同类型的乳腺癌细胞，研究miR-31对GNA13在蛋白水平和mRNA表达水平的影响以及GNA13蛋白相对表达量与细胞侵袭能力的相关性。结果在MCF-10A细胞中，pMSC-GNA13wt载体转染组GNA13 mRNA相对表达量（7.26±1.80）显著高于空载体转染组（1.05±0.02，P＝0.001）。pMSC-GNA13wt载体转染组细胞侵袭的相对数目[（13.35±0.47）个]显著高于空载体转染组[（1.38±0.12）个]细胞数目（P＝0.000），GNA13 mRNA表达水平与miR-31表达水平呈负相关性。结论通过减少GNA13表达可抑制miR-31对乳腺癌细胞侵袭。Objective To investigate the expression pattern and role of microRNA （miRNA, mill） -31 in breast cancer cells. Methods Transfected recombinant plasmid pMSC - G protein subunit alpha 13 （GNAI3） wt constructed by molecular cloning into different types of breast cancer cells. To study the expression of GNA13 at mRNA and protein level as well as the effect of GNA13 ectopic expression on cell invasion capacity. Results The relative expression level of GNA13 mRNA in pMSC - GNA13wt - transfected MCF - IOA cells （7. 26 ± 1.80） significantly higher than pMSC - c - transfected ceils （ 1.05 ± 0. 02, P = 0. 001 ）. Relative invasion cell in pMSC - GNA13wt - transfected MCF - 10A cells （ 13.35 ± 0. 47 ） significantly higher than pMSC - c - transfected cells （ 1.38 ±0. 12, P = 0. 000）. Examination of hu- man breast cancer tissues revealed that GNA13 mRNA levels were inversely correlated to rniR- 31 levels. Conclusion These data provide strong evidence that miR -31 regulates breast cancer cell invasion partially via targeting GNA13 expression in breast cancer cells. Loss of miR -31 expression could be used as biomarkers of breast cancer progression.

关 键 词：微小RNA-31 异源三聚体G蛋白13 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]