长链非编码RNA GAPLINC在人胃癌异时性肝转移组织表达及其临床意义  

Clinical significance of long chain noncoding GAPLINC in human gastric carcinoma with metachronous liver metastasis

作　　者：范钰[1] 金鑫[1] 蒋孟林 陶曙[2] 邹晨[2] 方娜[1] 

机构地区：[1]江苏大学附属人民医院肿瘤研究所,镇江212002 [2]江苏大学附属人民医院普外科,镇江212002

出　　处：《中华实验外科杂志》2017年第4期659-661,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省省级重点研发专项基金（BE2015666）;江苏省333工程科研基金（BRA2016140）;镇江市社会发展基金（SH2015050、SH2015061）;镇江市卫生科技重点专项项目（SHW2015004、SHW2015006）;江苏大学临床科技基金（JLY20160013、JLY20160014、JLY20160023、JLY20160025）

摘　　要：目的 探讨长链非编码RNA GAPLINC在人胃癌异时性肝转移中临床意义。 方法 收集37例胃癌及其配对正常胃黏膜组织，采用荧光实时定量聚合酶链反应（PCR）方法检测组织中GAPLINC表达水平，分析其表达与患者性别、年龄、肿瘤部位、肿瘤直径、组织分化程度、肿瘤浸润深度、淋巴结转移、血管浸润、神经浸润、肝转移数目及肝转移发生时间等临床病理因素的关系。结果 在37例胃癌组织中GAPLINC阳性率为89.19% （33/37），而正常胃组织GAPLINC阳性率为2.70% （1/37）。两者差异有统计学意义（χ2＝55.718，P＝0.000）。GAPLINC表达量与患者性别、年龄、肿瘤位置、组织分化程度均无相关性，但与原发灶直径（χ2＝6.578，P＝0.021）、肿瘤浸润深度（χ2＝9.291，P＝0.005）、淋巴结转移（χ2＝7.117，P＝0.040）、血管浸润（χ2＝11.407，P＝0.010）、神经浸润（χ2＝14.507，P＝0.005）、肝转移数目（χ2＝6.257，P＝0.038）、肝转移发生时间（χ2＝12.015，P＝0.002）均显著相关。说明GAPLINC与胃癌异时性肝转移密切相关。结论 GAPLINC可能是胃癌异时性肝转移重要靶点之一。Objective To investigate the clinical significance of long chain non coding RNA gastric adenocarcinoma associated, positive CD44 regulator, long intergenic non-coding RNA （GAPLINC） in human gastric cancer with metachronous liver metastasis. Methods Human gastric cancer and matched normal gastric tissue were collected, and GAPLINC was detected by fluorescence quantitative real-time polymerase chain reaction （PCR） assay. The relationship between GAPLINC expression and patient’s sex, age, tumor location, tumor diameter, histological differentiation, depth of tumor invasion, lymph node metastasis, infiltration of blood vessels, nerve infiltration, the number of liver metastases, the time of liver metastasis, and distant metastasis was analyzed, respectively.ResultsThe positive rate of GAPLINC in 37 cases of gastric cancer was 89.19% （33/37）, and that in normal gastric tissues was 2.70% （1/37） （χ2=55.718, P=0.000）. GAPLIC expression was not significantly correlated with the age and sex of patients, tumor location, histological differentiation, but with the primary tumor diameter （χ2=6.578, P=0.021）, depth of tumor invasion （χ2=9.291, P=0.005）, lymph node metastasis （χ2=7.117, P=0.040）, vascular invasion （χ2=11.407, P=0.010）, neural invasion （χ2=14.507, P=0.005）, the number of liver metastases （χ2=6.257, P=0.038）, and liver metastasis time （χ2=12.015, P=0.002）. Conclusion These results indicate that GAPLINC is closely correlated to gastric cancer with metachronous liver metastasis. GAPLINC might be one of the important targets of metachronous liver metastasis in gastric cancer.

关 键 词：胃肿瘤 肝转移 长链非编码RNA GAPLINC 

分 类 号：R735.2[医药卫生—肿瘤]