紧密连接蛋白Claudin-7在大肠癌及大肠腺瘤组织中的表达  

Expression of tight junction proteins Claudin -7 in colorectal carcinoma and colorectal adenoma

作　　者：马志杰[1] 朱志坚[1] 樊丽伟[1] 李元平[1] 曹伟[1] 刘艺[2] 王爱民[1] 

机构地区：[1]解放军第252医院消化科,河北保定071000 [2]解放军第252医院病理科,河北保定071000

出　　处：《中华实验外科杂志》2017年第4期666-668,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 检测紧密连接蛋白Claudin-7在正常大肠黏膜、不同病理分型的大肠腺瘤和不同分期的大肠癌组织中的表达，探讨其在大肠癌变过程中不同阶段表达的意义。方法 收集内镜下切除的不同病理分型的大肠腺瘤共80例，其中包括管状腺瘤30例，管状绒毛状腺瘤30例，绒毛状腺瘤20例；以及普通外科手术切除的大肠癌组织60例和癌旁正常组织40例，应用免疫组织化学方法检测组织中Claudin-7蛋白的表达。结果 Claudin-7在正常大肠组织中100%的表达，腺瘤和大肠癌中Claudin-7的阳性表达率明显降低，大肠癌组织中阳性表达率最低（46.7%），三者阳性表达率比较差异有统计学意义（P＝0.000）。其在大肠癌中表达与肿瘤的分期、组织分化程度、淋巴结转移明显相关。在大肠管状腺瘤、混合性腺瘤、绒毛状腺瘤中的表达阳性率分别为83.3%、63.3%、50.0%，差异有统计学意义（P＝0.040）。 结论 Claudin-7的表达对肿瘤的发生、发展起抑制作用，并且在大肠癌发生的早期出现表达的异常。To detect the expression of Claudin-7 protein in the normal mucosa, colorectal adenoma with different pathological types and colorectal carcinoma with different stages and study its implication in different stages of colon carcinogenesis. Methods Eighty cases of colorectal adenoma samples with different pathological types removed under endoscopy were collected, which included 30 cases of tubular adenoma, 30 cases of tubular villous adenoma, and 20 cases of villous adenoma. The samples also included 60 cases of colorectal carcinoma specimens and 40 cases of paracancerous normal tissue specimens resected surgically. Immunohistochemistry was applied to detect the expression of Claudin-7 protein in the tissues. Results The positive rate of Claudin-7 expression in normal mucosa was 100%, however, that in colorectal adenoma and colorectal carcinoma was significantly decreased, and that was the lowest in colorectal carcinoma （46.7%）. The difference had statistical signifycance （P=0.000）. The expression of Claudin-7 was associated with the Dukes staging, the degree of differentiation and the lymph node metastasis. The positive rate of Claudin-7 expression in tubular adenoma group, mixed pattern of adenoma group and villous adenoma group was 83.3%, 63.3% and 50.0%, respectively, with significant differences among them （P=0.040）. Conclusion The expression of Claudin-7 inhibited the occurrence and development of colorectal carcinoma, and appeared abnormal in early colorectal carcinoma.

关 键 词：大肠腺瘤 大肠癌 紧密连接蛋白 Claudin-7 

分 类 号：R735.34[医药卫生—肿瘤]