微小染色体维持蛋白2在结直肠腺瘤及结直肠癌组织的表达及临床意义  

The expression and significance of minichromosome maintenance protein 2 in colorectal adenoma and colorectal carcinoma

作　　者：汪景坤 李洵[1] 曹伟[1] 付晓霞[1] 贾树杰[1] 万灵燕[2] 王爱民[1] 

机构地区：[1]解放军第252医院消化科,河北保定071000 [2]解放军第252医院病理科

出　　处：《中华实验外科杂志》2017年第4期672-674,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测微小染色体维持蛋白2（MCM2）在正常结直肠黏膜、不同病理类型的结直肠腺瘤和结直肠癌组织中表达，探讨它们在预示结直肠腺瘤恶变的作用。方法 利用免疫组织化学方法及实时荧光定量聚合酶链反应（FQ-PCR）方法检测MCM2在80例结直肠腺瘤、60例结直肠癌以及60例正常结直肠黏膜组织中的表达。结果 MCM2在正常肠黏膜组、结直肠腺瘤组、结直肠癌组中表达之间均差异有统计学意义（P＝0.031），阳性表达率分别为16.7%、55.0%、80.0%；在管状腺瘤、混合性腺瘤、绒毛状腺瘤中表达差异亦有统计学意义（P＝0.022），阳性表达率分别为40.0%、65.0%、75.0%；FQ-PCR显示MCM2特性性扩增，MCM2在正常结直肠黏膜、结直肠管状腺瘤、结直肠混合性腺瘤、结直肠绒毛状腺瘤及结直肠癌组织中依次增高，其表达量分别为1.187±0.323、4.126±1.339、9.577±0.838、22.150±4.077、48.020±4.811，F＝55.512，P＝0.029。SNK多重比较结果显示任两组间总体均数差异有统计学意义。 结论 MCM2与结直肠腺瘤的恶变及结直肠癌的发生明显相关。Objective To explore the expression of minichromosome maintenance protein 2 （MCM2） in normal eoloreetal mueosa, eolorectal adenomas and colorectal carcinoma, and to analyze the relationships among different histological types of coloreetal adenomas and colorectal carcinoma. Methods Immunohistoehemistry was used to exzamine the location and expression of MCM2 in the colorectal tissues, colorectal adenoma tissues and colorectal carcinoma tissues. Real - time fluorescent quantitative polymerase chain reaction （FQ- PCR） was used to detect MCM2 mRNA and connexin- 32 mRNA expression. Re- suits （ 1 ） Immunohistochemistry： The positive expression rate of MCM2 protein in normal colorectal mu- cosa, colorectal adenoma and colorectal carcinoma tissues was 16. 7%, 55.0% and 80.0% respectively, with significant differences among them （P = 0. 031 ）. The positive expression rate of MCM2 in tubular ad- enoma, mixed pattern of adenoma and villous adenoma was 40. 0% , 65.0% and 75.0% respectively, also with significant differences among them （ P = 0. 022）. （2） FQ - PCR ： The MCM2 mRNA expression in the normal colorectal mucosa, tubular adenoma, the mixed pattern of adenoma, villous adenoma, and colo rectal carcinoma was 1. 187 ± 0. 923, 4. 126±1. 339, 9. 577 ± 0. 838, 22. 150 ± 4. 077 and 48. 020 ± 4. 811, respectively. The expression of MCM2 gradually increased in colorectal carcinoma, villous adenoma, the mixed pattern of adenoma, tubular adenoma, and normal colorectal mueosa （F = 55. 512, P = 0. 029）. SNK multiple comparisons displayed that the differences about any two groups of the total mean showed statistical significance. Conclusion MCM2 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma. Detecting the expression of MCM2 can evaluate the progres sion of eolorectal adenoma and the development of colorectal carcinoma. They may be the molecular biologi cal indicators for predicting caneeration of colorectal adenoma.

关 键 词：结直肠腺瘤 结直肠癌 免疫组织化学 微小染色体维持蛋白2 

分 类 号：R735.34[医药卫生—肿瘤]