胃癌患者血清IL-4、IFN-γ、TNF-α水平与病理特征的关系  

Correlation of serum levels of IL-4, IFN-γ and TNF-α with clinicopathologic features in patients with gastric cancer

作　　者：王凤梅[1] 李玲[1] 刘永琦[1,2,3] 伍志伟[1,3] 任春贞 卢志伟[1] 苏韫[1,3] 聂蓬 Feng-Mei Wang Ling Li Yong-Qi Liu Zhi-Wei Wu Chun-Zhen Ren Zhi-Wei Lu Yun Su Peng Nie(Gansu University of Chinese Medicine, Lanzhou 730000, Gansu Province, China Basic Medical College of Gansu University of Chinese Medicine, Lanzhou 730000, Gansu Province, China Key Laboratory for Translation of Dunhuang Medicine at Provincial and Ministerial Level, Lanzhou 730000, Gansu Province, China Gansu Wuwei Tumor Hospital, Wuwci 733000, Gansu Province, China)

机构地区：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃省兰州市730000 [2]甘肃中医药大学基础医学院,甘肃省兰州市730000 [3]敦煌医学与转化省部其建教育部重点实验室,甘肃省兰州市730000 [4]甘肃省武成肿瘤医院,甘肃省武威市733000

出　　处：《世界华人消化杂志》2017年第11期1006-1011,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目,No.81660744;甘肃省中医药管理局科研项目,No.GZK-2016-4~~

摘　　要：目的通过检测胃癌(gastric cancer,GC)患者血清白细胞介素-4(interleukin-4,IL-4)、干扰素-γ(interferon-γ,IFN-γ)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平,探讨其与GC病理特征的关系.方法酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测50例GC患者,50例正常对照者血清IL-4、IFN-γ、TNF-α水平并比较,同时获取GC患者的病理诊断资料,用spearman相关分析法分析IL-4、IFN-γ、TNF-α三者水平与GC临床病理特征的相关性.结果GC患者IL-4水平较正常对照组升高(57.62 vs42.63,P<0.05),TNF-α水平较正常对照组升高(315.08 vs 34.21,P<0.05),两者差异有统计学意义;GC患者IFN-γ水平较正常对照组降低(14.95 vs 24.92,P<0.05),差异有统计学意义.Spearman相关分析结果表明IL-4与GC的浸润深度呈正相关(r=0.571,P<0.05),IFN-γ与GC的浸润深度(r=-0.671,P<0.05)、淋巴结转移数量呈负相关(r=-0.526,P<0.05),TNF-α与肿瘤的分化程度呈正相关(r=0.816,P<0.05),三者与其他病理指标无相关(P>0.05).结论GC患者存在IL-4、IFN-γ、TNF-α表达紊乱,且三者变化与病理学特征密切有关,参与GC的发生、发展,对预测GC生物学行为及制定治疗策略有一定的临床价值.AIM To detect the serum levels of interleukin-4（IL-4）,interferon-γ（IFN-γ） and tumor necrosis factor-α（TNF-α） in gastric cancer（GC）patients and analyze their correlation with clinicopathologic features.METHODS A total of 50 GC patients and 50 healthy subjects were included in this study.We detected their serum levels of IL-4,IFN-y and TNF-α by enzyme-linked immunosorbent assay and collected their clinicopathological data.Then we analyzed the correlation between the serum levels of IL-4,IFN-y,TNF-α and the clinicopathologic features by spearman correlation analysis.RESULTS Compared to healthy subjects,serum levels of IL-4 and TNF-α significantly increased（57.62 pg/mL vs 42.63 pg/mL,P〈0.05;315.08 pg/mL vs 34.21 pg/mL,P〈0.05）,but serum level of IFN-y significantly decreased（14.95 pg/mL vs 24.92 pg/mL,P〈0.05） in GC patients.There was a positive correlation between serum level of IL-4 and the depth of tumor invasion（r = 0.571,P〈0.05）,but there was a negative correlation between serum level of IFN-y and the number of metastatic lymph nodes（r =-0.526,P〈0.05） and depth of tumor invasion（r =-0.671,P〈0.05）.Serum level of TNF-α was positively correlated with tumor differentiation（r = 0.816,P〈0.05）.CONCLUSION GC patients have altered serum levels of IL-4,IFN-y and TNF-α,and they may be related to the occurrence and development of GC,and have certain clinical value in predicting the biological behavior of GC and in developing treatment strategies. Provincia lLevel Key Laboratory for Molecular Medicine of Major Diseases and Study on Prevention and Treatment of Traditional Chinese Medicine,

关 键 词：胃肿瘤 细胞因子类 病理特征 

分 类 号：R735.2[医药卫生—肿瘤]