番茄与马铃薯TIFY家族基因的鉴定与比较分析  

Identification and Comparative Analysis of the TIFY Family Genes in Tomato and Potato

作　　者：胡利宗[1,2] 王俊生[1] 赵锦慧[1] 王秋霞[1] 韩娇月 纪秀娥[1] 

机构地区：[1]周口师范学院,生命科学与农学学院,周口466001 [2]周口师范学院,河南省作物分子育种与生物反应器重点实验室,周口466001

出　　处：《分子植物育种》2017年第4期1192-1203,共12页Molecular Plant Breeding

基　　金：河南省教育厅项目(17B180009);周口师范学院2014年高层次人才科研启动经费资助项目(ZKNU2014109);周口师范学院2015年度校本项目(ZKNUB215103)共同资助

摘　　要：TIFY蛋白是一类植物特有的转录因子,在植物的生长发育、信号传导以及胁迫反应中起重要作用。尽管一些模式植物TIFY家族基因已经被鉴定,但在茄科植物中有关TIFY家族基因的研究却很少。本研究利用生物信息学手段分别从番茄与马铃薯中挖掘到21个Sl TIFY和21个St TIFY基因,并系统性分析这些TIFY基因的序列、表达以及进化特性。染色体定位与扩增模式分析显示,番茄和马铃薯的TIFY基因散落分布在不同染色体上,家族成员扩增主要由串联和片段重复产生。序列分析表明:番茄TIFY基因结构被分为12种,马铃薯TIFY基因结构被分为8种;番茄和马铃薯TIFY蛋白共享了保守基序1,其它保守基序显示不同程度基因特异性,保守基序组成模式表现出高度多样化。系统进化分析显示,番茄和马铃薯TIFY蛋白被分为7大类群,同类群内结构与序列相似性较高。选择压力检测表明,番茄的10对同源基因和马铃薯的19对同源基因均受到负选择作用。表达谱结果表明,番茄和马铃薯TIFY基因的表达呈现较高的多样性,这可能与多样化的功能相关。这些研究结果为功能解析番茄和马铃薯TIFY基因提供理论指导。TIFY proteins are plant-specific transcription factors that play important roles in plant growth and development, signal transduction and stress response. Although some members of TIFY family genes were characterized in several model plants, the reports about TIFY family genes remain largely limited to the Solanaceae species. In this study, 21 SITIFY and 21 StTIFY genes were identified in both tomato and potato, respectively. In addition, sequence, expression, and evolution of the TIFY genes were further systematically analyzed using the bioinformatics methods. Chromosomal mapping and expansive pattern studies showed that the TIFY genes, generated by tandem and segmental duplication, were unequally distributed in tomato and potato chromosomes. Sequence analyses were performed on the TIFY proteins. 12 and 8 types of gene structures were identified in tomato and potato, respectively. The conserved Motif-1 was shared by all the TIFY proteins, but other conserved motifs showed different degree of gene specificity, the pattern of conserved motifs showed a high degree of diversification. TIFY proteins from tomato and potato were divided into seven groups by phylogenetic analysis. All the proteins within the same group share high similarity of structure and sequence. Selective pressure analysis indicated that 10 and 19 homologous gene pairs from tomato and potato, respectively, were under negative selection. The results showed that the expression of TIFY gene in tomato and potato showed high diversity, which may be related to the diversity of functions. These results provided theoretical guidance for the functional analysis of the TIFY gene in tomato and potato.

关 键 词：番茄 TIFY家族 基因 马铃薯 进化 

分 类 号：S532[农业科学—作物学] S641.2