试纸法快速测定保健品中茶多酚含量的研究  

Research on rapid determination of tea polyphenols in the health care products by using the test paper

作　　者：张少华[1] 霍丹群[1] 侯长军[1] 杨眉[1] 张良 

机构地区：[1]重庆大学生物工程学院,重庆400044 [2]泸州老窖集团有限责任公司,泸州646000

出　　处：《应用化工》2016年第12期2369-2372,共4页Applied Chemical Industry

基　　金：国家自然科学基金(31171684);国家科技支撑计划课题(2014BAD07B02);四川省院士(专家)工作站固态发酵关键技术研究(GY2015-01);四川省重点实验室项目(NJ2014-03);重庆大学大型仪器设备开放基金资助

摘　　要：提出了一种方便、快速、半定量、可视化的茶多酚试纸检测新方法。实验表明,在pH 7~8时,茶多酚与试纸上的酒石酸亚铁生成蓝紫色的三元络合物并且颜色深度和茶多酚含量成正比,之后通过与标准色阶对比即可得出茶多酚的含量。试纸可以在5 min之内实现对茶多酚含量的快速检测,最低检测限为0.005 g/L。通过和HPLC检测结果的对比,证明本试纸可以用于实时检测保健品中的茶多酚含量,检测结果具有较高的准确性和灵敏性。A new method for the convenient,rapid,semi-quantitative,and visual detection of tea polyphenols（ TPs） was demonstrated by using the test paper. The detecting principle of the test paper is based on the fact that the Ferrous tartrate can react with the tea polyphenols to generate violet ternary complexes,and the color depth of complexes is positively correlated with the TP concentration,and determine the TP content by comparing the color development results with the standard color gradation. The test paper can finish the detection within 5 min which the limit of detection is 0. 005 g/L. By comparing with the result of HPLC,the test paper is proved can be used in the real-time and rapid detection of the tea polyphenols in the health care products with high accuracy and sensitivity.

关 键 词：茶多酚 试纸 快速检测 

分 类 号：TQ203.3[化学工程—有机化工]