Rab27在膀胱癌细胞中过表达并促进细胞的增殖和侵袭  

Overexpression of Rab27 in bladder cancer and its promotion of cell proliferation and invasion

作　　者：刘嘉[1] 宫雪[1] 祝兴旺[1] 薛东炜[1] 刘屹立[1] 王平[1] 

机构地区：[1]中国医科大学附属第四医院泌尿外科,辽宁沈阳110032

出　　处：《现代肿瘤医学》2017年第11期1700-1704,共5页Journal of Modern Oncology

基　　金：辽宁省膀胱系统疾病转化医学研究中心及协同研究网络建设-膀胱癌转化医学研究项目(编号:2015225009)

摘　　要：目的:检测Rab27在人膀胱癌细胞系中的表达情况,并探讨Rab27表达与膀胱癌增殖侵袭的关系。方法:采用Western blot和RT-PCR检测Rab27在BIU-87、5637、RT4、J82和T24细胞系中的表达。应用Rab27转染人膀胱癌细胞系BIU-87,应用Rab27 siRNA干扰人膀胱癌细胞系5637后,应用CCK-8、集落形成实验和基质胶侵袭实验来检测Rab27对膀胱癌增殖和侵袭的影响。结果:CCK-8、集落形成实验结果表明膀胱癌细胞系中Rab27转染能够提高细胞的增殖率,而Rab27敲除后则抑制细胞的增殖。基质胶侵袭实验表明Rab27敲除后下调了穿过基底膜的细胞数,而转染Rab27后穿过基底膜的细胞数显著增多。结论:Rab27促进着膀胱癌细胞的增殖与侵袭能力。Objective To detect the expression of Rab27 in human bladder cancer cell line , and to explore the re-lationship between Rab27 expression and the proliferation and invasion of bladder cancer. Methods ： Western blot and RT -PCR were performed to detect the expression of Rab27 in BIU -87,5637,RT4,J82 and T24 bladder cancer cell line. After Rab27 was transfected into human bladder cancer cell line BIU -87,and the siRNA Rab27 was used to in-terfere with the human bladder cancer cell line 5637, the effect of Rab27 on the proliferation and invasion of bladder cancer was detected by CCK - 8 ,colony forming assay and matrix gel invasion assay. Results ： CCK - 8 , colony forma-tion assay showed that Rab27 transfection in bladder cancer cell line could improve the cell proliferation rate,while Rab27 knock down inhibited cell proliferation. Matrix gel invasion assay showed that Rab27 knock down reduced the number of cells through the basement membrane, and the number of cells passing through the basement membrane was significantly increased after transfection with Rab27. Conclusion ： Rab27 promotes the proliferation and invasion of bladder cancer cells.

关 键 词：Rab27 膀胱癌 增殖 侵袭 

分 类 号：R737.14[医药卫生—肿瘤]