固绿褪色反应体系分光光度法测定食品中丙二醛  

Determination of malondialdehyde in food by color-fading reaction of fast green-spectrophotometry

作　　者：杨秀军 马璐 凌绍明[3] 

机构地区：[1]广西百色市粮油质量监督检验中心,广西百色533099 [2]百色学院预科教育学院,广西百色533099 [3]百色学院化学与环境工程,广西百色533099

出　　处：《粮油食品科技》2017年第3期61-64,共4页Science and Technology of Cereals,Oils and Foods

摘　　要：在硫酸介质中,Fenton反应产生的羟基自由基使固绿褪色,丙二醛(MDA)对羟基自由基氧化固绿的反应具有抑制作用,据此提出了测定MDA含量的固绿褪色—分光光度法。在最优的实验条件下,方法的线性范围为0.083~3.33μg/mL,回归方程为△A_(625nm)=0.083 7ρ+0.020 4,相关系数为0.992 6,检出限为0.038μg/mL。方法应用于食品中MDA的测定,测定结果的RSD为1.5%~2.7%,回收率在96.2%~106.9%。In sulfuric acid solution, Fenton reaction was used to produce hydroxyl radical to discolor fast green. But malondialdehyde（ MDA ） could inhibit the oxidation of hydroxyl radical on fast green. Based on this fact, spectrophotometric method for determination of MDA by color - fading of fast green was pro-posed. In the optimal experimental conditions, in the linearity range of 0. 083μg / m L -3. 33 μg/m L , the regression equation was Δ 625n m =0. 083 7ρ ＋0. 020 4 ,with correlation coefficient of 0. 992 6 and detection limit of 0. 038μg/mL . For the determination of MDA in foods, this method presented R S D of 1.5% -2.7% and recovery of 9 6.2%-106.9%.

关 键 词：分光光度法 丙二醛 褪色反应 固绿 

分 类 号：TS207.3[轻工技术与工程—食品科学]