神经吻合包埋鞘内灌注神经生长因子对大鼠坐骨神经损伤修复的影响  

Effect of nerve growth factor by neurilemma perfusion on recovery of rat sciatic nerve function in neural anastomosis

作　　者：王韦江[1] 王园园[2] 范金鹏[1] 王朝君[1] 张春霞[1] 

机构地区：[1]河北省石家庄市第一医院,河北石家庄050011 [2]河北省石家庄市第三医院,河北石家庄050011

出　　处：《现代中西医结合杂志》2017年第16期1718-1721,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省医学科研重点课题计划项目(20150160)

摘　　要：目的研究神经生长因子不同给药方式对大鼠坐骨神经损伤修复的影响。方法采用切断一侧坐骨神经方法造成大鼠神经损伤模型,将术后30只SD大鼠随机分为3组,每组10只。神经生长因子灌注组于神经吻合口包埋鞘内灌注神经生长因子(30μg/2mL)0.1 mL/h,神经生长因子肌注组肌肉注射神经生长因子30μg/d,生理盐水灌注组于神经吻合口包埋鞘内灌注等量生理盐水,均连用1周。术后第2,4周观察动物行为学表现,第4周行患肢足迹分析,计算神经功能指数,然后行神经电生理检查。结果术后4周,各组大鼠足部创面基本愈合,神经生长因子灌注组和神经生长因子肌注组大鼠伤肢步态基本正常,生理盐水灌注组大鼠步态仍未明显恢复,3组大鼠展爪反射均未见明显恢复。神经生长因子灌注组和神经生长因子肌注组患侧足印测量指标均较生理盐水灌注组更接近于正常(P均<0.05),SFI、TFI、PFI均明显高于生理盐水灌注组(P均<0.05);且神经生长因子灌注组除SFI与神经生长因子肌注组比较差异均无统计学意义(P均>0.05)外,其余指标改善情况均明显优于神经生长因子肌注组(P均<0.05)。神经生长因子灌注组神经传导速度明显快于其他2组(P均<0.05),潜伏期明显短于其他2组(P均<0.05);神经生长因子肌注组神经传导速度和潜伏期与生理盐水灌注组比较差异均无统计学意义(P均>0.05)。结论神经吻合包埋鞘内灌注神经生长因子可明显促进坐骨神经功能恢复。Objective It is to study the effect of nerve growth factor （ NGF） by different dosing on recovery of rat sciatic nerve function in neural anastomosis. Methods The rat models of nerve injury were established by cutting off their sciatic nerve on one side,then the successful models of 30 SD rats were divided into 3 groups, each group had 10 rats. NGF perfusion group was given NGF （30 μg/2mL） 0. 1 mL/h by neurilemma perfusion, NGF muscle injection group was given NGF 30 μg/ d by muscle injection, normal saline （NS） perfusion group was given NS at the same dose by neurilemma, all the three groups were treated for one week. The behavior of rats after 2 and 4 weeks’treatment were recorded,the limb footprint analysis was performed on after 4 weeks ’ treatment,never functional index was calculated, neuroelectrophysiologic examination was per-formed. Results In 4 weeks after operation,wound surface of the rats in every group almost healed,the rats had normal gait in NGF perfusion group and NGF muscle injection group, but gait of the rats in NS perfusion group had no significant recovery. No obvious recovery of claw stretching reflection was found in the 3 groups. Limb footprint analysis and never function index showed that the index in NGF perfusion group and muscle injection group were more closely with normal value and SFI, TFI, PFI were higher than that in NS perfusion group（ P 〈 0.05 ） , and the improvements of these indexes except SFI were better in NGF perfusion group than that in NS perfusion group （P〈0 0. 05） . Never conductive velocity was faster while latency was shorter in NGF perfusion group than that in the other 2 groups （P〈00. 05） . There was no significant difference in never conductive velocity and latency between NGF perfusion group and NS perfusion group. Conclusion Nerve growth factor by neurilemma perfusion can improve the sciatic nerve functional recovery obviously.

关 键 词：神经生长因子 坐骨神经 灌注 神经功能指数 神经损伤 

分 类 号：R-332[医药卫生]