TGF-β1对卵巢癌细胞A2780增殖及迁移侵袭的影响  

Effect of TGF-β1 on the Proliferation, Migration and Invasion of Ovarian Cancer Cell Line A2780

作　　者：陈雨龙[1] 宋克娟[2] 曹佃霞 吕腾[2] 于晓敏[3] 姚勤[2] 

机构地区：[1]青岛大学,山东青岛266071 [2]青岛大学附属医院妇科,山东青岛266003 [3]青岛市第八人民医院妇科,山东青岛266041

出　　处：《现代生物医学进展》2017年第14期2627-2630,2615,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81100697/H1208);山东省博士基金项目(BS2011SW003)

摘　　要：目的:探讨TGF-β1对卵巢癌细胞A2780增殖、迁移及侵袭能力的影响。方法:以体外培养的卵巢癌细胞A2780为研究对象,给予不同浓度(0、2、4…20 ng/m L)TGF-β1处理不同时间(12、24…72 h)。采用CCK-8法检测不同的浓度TGF-β1处理不同时间对卵巢癌细胞A2780增殖的影响。根据增殖实验结果选择合适的TGF-β1作用浓度及处理时间,采用细胞划痕实验测定细胞的迁移能力,Transwell实验检测细胞的侵袭及迁移能力。结果:相较于空白对照组,TGF-β1可以剂量和时间依赖性显著促进卵巢癌细胞A2780的增殖(P<0.05)。细胞划痕实验结果显示TGF-β1处理组ΔS%/h明显高于空白对照组(P<0.05);Transwell迁移实验结果显示:TGF-β1处理组OD570明显高于空白对照组,差异具有统计学意义(P<0.05)。Transwell细胞侵袭实验结果显示:与空白对照组相比,TGF-β1处理组OD570明显升高(P<0.05)。结论:TGF-β1可以明显促进卵巢癌细胞系A2780的增殖、迁移及侵袭能力,其促增殖效应呈剂量/时间依赖效应。Objective： To investigate the effect of TGF-β1 on the proliferation, migration and invasion of ovarian cancer cell line A2780. Methods： Ovarian cancer cell line A2780 were treated with different concentration （0, 2, 4…20 ng/mL） of TGF-β1 for different time （12, 24…72 h） in vitro. CCK-8 cell counting assay was used to detect the proliferation ability. A proper treatment concentration and time was chosen depending on the proliferation assay results, then wound healing assay was used to examine the cell migration ability and transwell assay was employed to determine the migration and invasion ability of ovarian cancer cell line A2780. Results： Compared with the blank control group, TGF-β1 could significantly promote the proliferation of ovarian cancer cell line A2780 in a dose-/time-dependent manner（P〈0.05）. The wound healing assay showed that the △S%/h of TGF-β1 treated group was obviously higher than that of the blank control group （P〈0.05）. The Transwell migration assay showed that the OD570 of TGF-β1 treated group was higher than that of the blank control group（P〈0.05）. Compared with the blank control group, transweU invasion assay showed that the OD570 of TGF-β1 treated group was much higher （P〈0.05）. Conclusions： TGF-β1 could significantly promote the proliferation of ovarian cancer cell line A2780 in a dose-/time-dependent manner. It could also enhance the migration and invasion ability of ovarian cancer cell line A2780.

关 键 词：TGF-Β1 卵巢癌 增殖 迁移 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]