组蛋白分子伴侣FACT促进DNA复制偶联的核小体组装  

The Histone Chaperone FACT Contributes to DNA Replication-Coupled Nucleosome Assembly

作　　者：Jiayi Yang Xu Zhang Jianxun Feng He Leng Shuqi Li Junyu Xiao Shaofeng Liu Zhiyun Xu Jiawei Xu Di Li Zhongshi Wang Jingyang Wang 李晴[2] 

机构地区：[1]不详 [2]北京大学生命科学学院北京大学-清华大学生命科学联合中心

出　　处：《科学新闻》2017年第4期136-136,共1页Science News

基　　金：北京大学-清华大学生命联合中心;国家自然科学基金委的支持

摘　　要：真核生物DNA复制时，复制叉前的核小体解聚以允许复制体前行，同时在新合成的DNA子链上新的核小体必须马上组装，这被称为DNA复制偶联的核小体组装，是染色质复制的关键一步。DNA replication-coupled (RC) nucleosome assembly is mediated by histone chaperones and is fundamental for epigenetic inheritance and maintenance of genomic integrity. The mechanisms that promote this process are only partially understood. Here, we show that the histone chaperone FACT (facilitates chromatin transactions), consisting of Spt16 andPob3, promotes newly synthesized histone H3-H4 deposition. We describe an allele of Spt16 (spt16-m) that has a defect in binding to H3-H4 and impairs their deposition onto DNA. Consistent with a direct role for FACT in RC nucleosome assembly, spt16-m displays synthetic defects with other histone chaperones associated with this process, CAF-1 and Rtt106. Importantly, we show that FACT physically associates with Rtt106 and that the acetylation of H3K56, a mark on newly synthesized H3, modulates this interaction. Therefore, FACT collaborates with CAF-1 and Rtt106 in RC nucleosome assembly.

关 键 词：DNA复制 组装 FACT 分子伴侣 蛋白 染色质 

分 类 号：Q523[生物学—生物化学]