瑞芬太尼聚己内酯对脊髓缺血再灌注中神经细胞线粒体损伤的影响  

Effects of Remifentanil-poly-caprolactone on Neural Mitochondrial Injury during Spinal Cord Ischemia-Reperfusion

作　　者：张竞超[1,2] 李华凤[3] 方华[1,2] 张伟晶[1,2] 杨淼[1,2] 章放香[1,2] 王儒蓉[4] 刘进[4] 王泉云[4] 

机构地区：[1]贵州省人民医院麻醉科,贵阳550002 [2]贵州医科大学附属人民医院麻醉科,贵阳550002 [3]四川大学华西第二医院麻醉科,成都610041 [4]四川大学华西医院麻醉科,成都610041

出　　处：《四川解剖学杂志》2016年第4期7-10,共4页Sichuan Journal of Anatomy

基　　金：国家科技支撑计划课题资助(2014BAI05B05);贵州省科技厅基金资助项目(黔科合LH字[2014]7021号)

摘　　要：目的观察腹主动脉内灌注瑞芬太尼聚己内酯(REM-PCL)对兔脊髓缺血再灌注中神经细胞线粒体损伤的影响。方法 20只健康新西兰大白兔随机分为对照组(C组)及REM-PCL组(RP组,0.1mg/kg),每组10只。采用肾下腹主动脉阻断法,建立脊髓缺血再灌注损伤(SCIRI)模型。分别于缺血前、缺血45min、再灌注30min和60min时,测定脊髓神经细胞线粒体超氧化物歧化酶(SOD)、活性氧(ROS)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)、总抗氧化能力(T-AOC)、线粒体肿胀度(MSD),并观察其病理学改变。结果与缺血前比较,C组阻断腹主动脉后脊髓神经细胞线粒体SOD、GSH-PX及T-AOC均降低(P<0.01),ROS、MDA和MSD含量均升高;开放腹主动脉后C组脊髓神经细胞线粒体SOD、GSH-PX及T-AOC均降低(P<0.01),ROS、MSD和MDA含量均升高(P<0.01),脊髓灰质病理损害严重(P<0.01);RP组在阻断腹主动脉45min时和开放腹主动脉后脊髓神经细胞线粒体SOD、GSH-PX及T-AOC均显著高于C组(P<0.01),ROS、MSD和MDA含量均显著低于C组(P<0.01),RP组脊髓灰质的病理损害程度明显轻于C(P<0.01)。结论 SCIRI中腹主动脉内灌注REM-PCL可提高神经细胞线粒体抗氧化能力,减轻神经细胞线粒体损伤。Objective This study aimed to observe the effect of remifentanil-poly-caprolactone （REM-PCL）on neu ral mitochondrial injury during spinal cord ischemia-reperfusion. Methods The model of spinal cord ischemia-reperfusion injury （SCIRI） was performed by clamping the infrarenal aortic in twenty healthy New Zealand white rabbits, which were randomly divided into control group（physiological saline, group C, n= 10） and REM-PCL group（0. 1 mg？ kg^-1,group RP, n= 10）. Neural mitochondrial were isolated before ischemia,45 min after ischemia, 30 min and 60 min after reperfusion for determination of superoxide dismutase（SOD）, reactive oxygen species（ROS）, glutathione peroxidase （GSH-PX）, malondialdehyde （MDA）, total anti-oxidation capacity（T-AOC）,mitochondrial swelling degree（MSD）, and the pathological changes were investigated. Results The neural mitochondrial SOD, GSH-PX and T-AOC were decreased while ROS, MDA and MSD were increased after clamping of the abdominal aorta as compared to the value before ischemia in C group（P〈0.01）. SOD,GSH-PX and T-AOC were decreased while ROS, MDA and MSD were increased after declamping of the abdominal aorta as compared to the value before ischemia in C group（P 〈0.01）. The neural mitochondrial SOD, GSH-PX and T-AOC were distinctly higher while ROS, MDA and MSD were distinctly lower at 45min after clamping of the abdominal aorta and after declamping of the abdominal aorta in group RP than those in group C（P〈0.01）. The change of pathological damage to the gray matter of spinal cord were more serious in C and RG group during reperfusion than those in group RP （P〈0.01）. Conclusions The intra-aortic REM-PCL infusion could distinctly improve neural mitochondrial anti-oxidation capacity and attenuate neural mitochondrial injury during spinal cord ischemia-reperfusion injury（SCIRI）.

关 键 词：脊髓缺血再灌注损伤 阿片受体 线粒体 瑞芬太尼聚己内酯 

分 类 号：R744[医药卫生—神经病学与精神病学]