STIM1对乳腺癌细胞移动的作用及机制  

The mechanism research of STIM1 in breast cancer cells

作　　者：吴冰[1] 林天骥 阮仕娟 王斌[1] 邹飞[1] 

机构地区：[1]南方医科大学公共卫生学院职业卫生与职业医学系,广州市510515

出　　处：《实用医学杂志》2017年第9期1373-1376,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81101611)

摘　　要：目的:研究STIM1在乳腺癌细胞移动中的作用及分子机制。方法:Western blot检测STIM1 Si RNA序列干扰效率;Transwell检测沉默STIM1的表达及SOCE钙信号对细胞迁移的影响;钙图分析STIM1与SOCE钙信号的关系;Western blot检测分析沉默STIM1对FAK表达及激活的影响。结果:沉默STIM1表达抑制MDA-MB-231乳腺癌细胞的迁移;MDA-MB-231细胞中存在STIM1介导的SOCE;阻断SOCE抑制乳腺癌细胞的迁移;沉默STIM1不影响MDA-MB-231细胞的FAK表达及FAK的激活。结论:在乳腺癌细胞中,STIM1通过SOCE调控细胞内钙信号而影响细胞移动,然而,STIM1调控乳腺癌细胞移动与FAK的表达或激活无关。Objectives To explore the calcium signaling mechanism of STIM 1 in breast cancer cells. Methods After SiRNA interruption, Western blot and Transwell were used to measure protein expression of STIM 1 and cell migration in MDA-MB-231 cells respectively. The relationship between STIM1 and SOCE calcium signaling were analysed by Laser confocal microscopy. Western blots were used to measure protein expression of FAK after si- lence STIM1. Results The numbers of cells without STIM1 were significantly lower than those cells with STIM1 by Transwell assay. STIM1 mediated SOCE in MDA-MB-231. Blocking SOCE might inhibite cells migration. Silence STIM1 did not affect the expression or activation of FAK in MDA-MB-231 cells. Conclusion STIM1 influences cell migration through SOCE pathway in breast cancer cells, which is independent on the expression or activation of FAK.

关 键 词：乳腺癌细胞 STIM1 钙信号 FAK 

分 类 号：R737.9[医药卫生—肿瘤]