miR-9对肾癌细胞株侵袭和增殖能力的影响  

Effects of miR-9 on the invasion and proliferation of renal cancer cell line ACHN

作　　者：胡佳[1,2,3] 金露[2,3] 何韬[1,2,3] 赖裕林 全晶[2,3] 潘翔[2,3] 陈佩杰[2,3] 桂耀庭 来永庆[2] 倪梁朝[2] 

机构地区：[1]广州医科大学研究生院,广东广州510182 [2]北京大学深圳医院泌尿外科,广东深圳518036 [3]广东省男性生殖与遗传重点实验室,广东深圳518036

出　　处：《现代泌尿外科杂志》2017年第5期376-380,共5页Journal of Modern Urology

基　　金：国家自然科学基金(No.81101922);广东省自然科学基金(No.S2012010008365)

摘　　要：目的研究miR-9在肾癌、癌旁组织表达情况及其在肾癌细胞系(ACHN)中的作用。方法运用荧光定量RT-PCR方法测定32例肾癌及相应癌旁组织中miR-9的相对表达量。运用侵袭实验(transwell)及增殖实验(MTT、CCK-8)评估miR-9对ACHN功能的影响。结果与癌旁正常组织相比,miR-9在肾癌组织中表达显著上调(P<0.05)。体外实验证实,miR-9抑制物inhibitor可显著降低ACHN的侵袭及增值能力,而miR-9类似物minic可显著增加ACHN的侵袭及增值能力(P<0.05)。结论miR-9影响ACHN的侵袭及增殖能力,其表达可能与肾癌的发生及转移机制相关。ObjectiveTo explore the role of miR-9 in renal cancer, adjacent normal tissue and cell line （ACHN）.MethodsThe miR-9 expression in 32 cases of renal cancer was detected with RT-PCR.The effects of miR-9 expression on ACHN were determined with Transwell assay, CCK-8 and MTT assay.ResultsCompared with adjacent normal tissue, in renal cancer, miR-9 was significantly up-regulated （P〈0.05）.The miR-9 inhibitor significantly decreased the invasion and proliferation of ACHN, while miR-9 minic significantly increased the invasion and proliferation （P〈0.05）. ConclusionmiR-9 can affect the capacity of invasion and proliferation of ACHN, and it may lead to the pathogenesis and metastasis of renal cancer.

关 键 词：MIR-9 肾癌 ACHN 侵袭 增值 

分 类 号：R737.11[医药卫生—肿瘤]