比格犬肝门静脉置管并体外留置模型的建立  

Establishment of an animal model by placing one end of PICC in hepatic portal vein of a Beagle dog and leaving the other end out of its body

作　　者：孙百胜[1] 薛峥[2] 何跃忠[3] 法云智[4] 邱业峰[4] 杨展[1] 张雷[1] 裴禹皓 

机构地区：[1]军事医学科学院附属医院,北京100071 [2]航天中心医院急诊科,北京100049 [3]军事医学科学院科技部,北京100850 [4]军事医学科学院实验动物中心,北京100071

出　　处：《军事医学》2017年第4期310-312,共3页Military Medical Sciences

基　　金：国家科技支撑计划项目(2012BAI38B04)

摘　　要：目的建立比格犬肝门静脉置管并体外留置模型。方法将6只比格犬行气管插管吸入麻醉,取右腹直肌切口,定位肠系膜上静脉及肝门静脉主干,游离、切断肠系膜上静脉左侧属支,将中心静脉导管(PICC)插入并置于肝门静脉主干内,结扎固定。导管末端经右侧腹壁皮下引至颈背部穿出并固定。结果实验组6只比格犬麻醉效果良好,手术全部成功,平均手术时间约1 h,平均出血量约15 ml,术后5~7 d手术切口愈合。结论比格犬肝门静脉置管并体外留置模型的成功建立,可提高肝脏的靶向性用药药效、减少用药剂量、降低治疗或实验成本并减少药物不良反应;通过置管可直接采集肝门静脉血测得口服药物或毒物在首过消除之前的血药浓度,间接计算胃肠吸收率,为开展相关实验研究提供技术支持。Objective To establish an animal model by placing one end of PICC in the hepatic portal vein of a beagle dog and leaving the other end out of its body.Methods Six Beagle dogs were given respiration anesthesia through orotracheal intubation.An incision was made through the right rectus abdominalis to locate the superior mesenteric vein（SMA） and the main hepatic portal vein.The left branch of SMA was separated and cut to put PICC into the main hepatic portal vein before being ligated and fixed.The other end of PICC was elicited through the right abdominal wall and passed beneath the skin to the back neck and fastened in case of movement.Results The anesthetic effect was good and all the operations were successful.The mean operation time was about an hour and the mean blood loss was about 15 ml.The incision healed 5-7 d after operation.Conclusion The establishment of the model can improve the effects of livertargeting drugs,which can cut down the dosage,lower the cost of treatment and experiment and reduce the adverse effect of medicines.Through PICC,we can directly draw blood from the hepatic portal vein to measure the blood concentration before the first pass elimination.Then according to the concentration,we can calculate the absorption rate in the gastrointestinal tract,which can facilitate related experimental studies.

关 键 词：比格犬 吸入麻醉 动物模型 肝门静脉 

分 类 号：R-332[医药卫生]