延边黄牛γ-干扰素基因的克隆与序列分析  

Cloning and sequence analysis of IFN-γ gene in Yanbian bovine

作　　者：尹昌善 张立霞[2] 薛书江[2] 宋建臣[2] 李海峰[2] 许应天[2] 

机构地区：[1]和龙市动物检疫站,吉林和龙133500 [2]延边大学农学院,吉林延吉133002

出　　处：《延边大学农学学报》2017年第1期83-86,共4页Agricultural Science Journal of Yanbian University

基　　金：吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2015]第16号)

摘　　要：应用刀豆素蛋白(ConA)和植物分裂素(PHA)双重诱导培养延边黄牛脾淋巴细胞,提取脾淋巴细胞总RNA,RT-PCR扩增延边黄牛γ-干扰素基因,并将其克隆到pMD-18-T simple载体中,进行PCR鉴定和酶切鉴定及测序。结果表明:成功克隆了462bp大小的延边黄牛γ-干扰素基因片段;所克隆基因片段序列与欧洲牛、印度牛的亲缘关系最近,同源性分别为99.8%和99.6%,与欧洲牛的氨基酸同源性为100%。本试验为进一步研究延边黄牛γ-干扰素生物学作用奠定了基础。The Yanbian cattle IFN-γ gene was amplified by RT-PCR from total RNA of Yanbian cattle spleen lymphocytes double induced and cultured by ConA and PHA. IFN-γ gene was cloned into PMD-18- T simple vector,and operated on PCR identification and restriction enzyme digestion and sequencing. The results showed that Yanbian cattle IFN-γ gene was successfully cloned; The Yanbian cattle IFN-γgene was closest with European cattle IFN-γ gene and Indian cattle IFN-γ gene in genetic relationship, and the homology of genetic sequence were 99.8% and 99.6%. In addition, the homology of amino acid was 100% between Yanbian cattle IFN-γ＇ gene and European cattle IFN-γ gene. The experiment laid the foundation for the further study of biological effects of Yanbian cattle IFN-γ.

关 键 词：延边黄牛 Γ-干扰素基因 克隆 序列分析 

分 类 号：S823[农业科学—畜牧学]