气质联用测定卷烟主流烟气中的3-羟基吡啶  

Determination of Pyridin-3-ol in Mainstream Smoke of Cigarettes by GC-MS

作　　者：王加忠 赖东辉 蔡元青 毛寒冰 魏建科 罗熹 包亮 云雪 蒋惠智 刘剑 

机构地区：[1]贵州中烟工业有限责任公司技术中心,贵州贵阳550009

出　　处：《安徽农业科学》2017年第12期64-66,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究卷烟主流烟气粒相物中3-羟基吡啶含量,为卷烟感官评吸提供参考。[方法]采用N-甲基-N-三甲基硅烷三氟乙酰胺(MSTFA)-气相色谱-质谱分析化学手段,建立了3-羟基吡啶快速、高效、可靠的分析方法。该方法是用剑桥滤片采集卷烟烟气粒相物加内标(苯酚-D5)后用三氯甲烷进行超声萃取,衍生化后的萃取液进行气相色谱-质谱联用(GC-MS)分析。[结果]3-羟基吡啶衍生物能在较短时间内出峰,色谱峰型较好,检出限(3S/N)为31 ng/支,加标回收率在97.3%~98.1%,测定值的相对标准偏差(n=6)在1.5%~2.9%。[结论]该方法适用于卷烟主流烟气中3-羟基吡啶的快速分析。[ Objective] To study the content of pyridin-3-ol in the particulate phase of mainstream cigarette smoke and provide reference for cigarette sensory evaluation. [ Method] A rapid, efficient and reliable method for the determination of pyridin-3-ol was established by adopting N-methyl-N-（trimethylsilyl） trifluoroaeetamide （MSTFA） Silanization-GC-MS. The method is to use the Cambridge filter collection cigarette smoke particulate and adding internal standard （Phenol-D5） after extracted ultrasonically with chloroform, derivatization and extraction liquid after GC-MS analysis. [ Result] The results showed that： The pyridin-3-ol derivatives can be produced in a short period of time, good chroma- tographic peak type, detection limit （3S/N） of the method found was 31 ng/cig, values of recovery found by standard addition method were in the range of 97.3 %-98.1% , and values of RSD＇s （n = 6） were found in the range of 1.5%-2.9%. [ Conclusion ] This method is suitable for the rapid analysis of pyridin-3-ol in mainstream cigarette smoke.

关 键 词：3-羟基吡啶 气相色谱-质谱 主流烟气 衍生化 

分 类 号：TS411[农业科学—烟草工业]