早期肺癌患者血清中MIF、IGFBPs含量与病灶中癌细胞活力的相关性  

Correlation of serum MIF and IGFBPs levels with the cancer cell viability in the focus of patients with early lung cancer

作　　者：李会广 高贵荃 

机构地区：[1]陕西省铜川矿务局中心医院核医学科,陕西铜川727000 [2]辽宁省鞍山市中医医院检验科,辽宁鞍山114000

出　　处：《海南医学院学报》2017年第7期987-989,993,共4页Journal of Hainan Medical University

基　　金：陕西省科学技术发展项目(SXBBE-248)~~

摘　　要：目的:研究早期肺癌患者血清中MIF、IGFBPs含量与病灶中癌细胞活力的相关性。方法:选择在我院手术切除的38例早期非小细胞肺癌患者作为肺癌组,同期体检的60例健康志愿者作为对照组。采集两组受试者的血清并测定MIF、IGFBPs的含量,采集肺癌组的肺癌组织和癌旁组织并测定SIRT-1相关分子的mRNA表达量。结果:肺癌组血清中MIF的含量显著高于对照组,IGFBP-3的含量显著低于对照组,IGFBP-2、IGFBP-5的含量与对照组比较无显著性差异;肺癌组织中SIRT-1、Vimentin、Ncadherin的mRNA表达量均显著高于癌旁组织,ZO-1、E-cadherin的mRNA表达量均显著低于癌旁组织;MIF高含量肺癌患者以及IGFBP-3低含量肺癌患者病灶组织中SIRT-1、Vimentin、N-cadherin的mRNA表达量较高,而ZO-1、E-cadherin的mRNA表达量较低。结论:早期肺癌患者血清中MIF的含量异常升高、IGFBP-3的含量异常降低,MIF和IGFBP-3含量的变化与SIRT-1所介导的癌细胞生长过程改变有关。Objective：To study the correlation of serum MIF and IGFBPs levels with the cancer cell viability in the focus of patients with early lung cancer.Methods：A total of 38 patients with early non-small cell lung cancer who received surgical resection in our hospital between May 2013 and August 2016 were selected as the lung cancer group,and 60 healthy volunteers who received physical examination during the same period were selected as the control group.The serum was collected from two groups of subjects to determine the MIF and IGFBPs levels,and the lung cancer tissue and the tissue adjacent to carcinoma were collected from the lung cancer group to determine the mRNA expression of SIRT-1-related molecules.Results：Serum MIF level of lung cancer group was significantly higher than that of control group,IGFBP-3level was significantly lower than that of control group,and the IGFBP-2and IGFBP-5levels were not significantly different from those of control group;SIRT-1,Vimentin and N-cadherin mRNA expression in lung cancer tissue were significantly higher than those in the tissue adjacent to carcinoma while ZO-1and E-cadherin mRNA expression were significantly lower than those in the tissue adjacent to carcinoma;SIRT-1,Vimentin and N-cadherin mRNA expression were higher while ZO-1and E-cadherin mRNA expression were lower in focus tissue of lung cancer patients with high MIF levels and those with low IGFBP-3levels.Conclusions：Serum MIF level increases abnormally while IGFBP-3level decreases abnormally in patients with early lung cancer,and the change of MIF and IGFBP-3levels is correlated with the change of cancer cell growth process mediated by SIRT-1.

关 键 词：非小细胞肺癌 巨噬细胞抑制因子 胰岛素生长因子结合蛋白 沉默信息调节因子-1 上皮间质转化 

分 类 号：R734.2[医药卫生—肿瘤]