呼气丙酮用于糖尿病肾病患者早期诊断的探讨  

Exhaled acetone is beneficial to early diagnosis of diabetic nephropathy

作　　者：臧宁[1] 黄燕文[1] 岑美婷[1] 臧诗蕾 

机构地区：[1]广州市第一人民医院南沙医院检验科,广州511457

出　　处：《广州医药》2017年第3期26-28,33,共4页Guangzhou Medical Journal

基　　金：广州市医药卫生科技一般引导项目西医类(20151A010014)

摘　　要：目的探讨呼出气体中丙酮能否用于糖尿病肾病的早期诊断。方法对健康对照组、2型糖尿病组(T2MD)和糖尿病肾病组(DN)分别采用气相色谱-质谱(GC-MS)法测定呼气丙酮含量、全自动生化仪的乳胶透射免疫比浊法测定尿微量白蛋白、酶速率法测试β-羟丁酸以及全自动尿液分析仪干化学法测定尿酮体,并对上述结果进行分析。结果在T2MD组和DN组中呼气中的丙酮含量均高于健康对照组(P<0.01);在DN组,丙酮与尿微量白蛋白水平呈线性相关(r=0.79,P<0.05)。结论检测呼气中的丙酮有助于糖尿病肾病的早期诊断,能及时反映病人肾脏损伤情况。Objective To explore the early diagnosis of diabetic nephropathy by exhaled acetone. Methods Respectively using GC-MS method for the determination of exhaled acetone content, emulsion transmission turbidimetric immunoassay method in automatic biochemical analyzer for determination of urinary microalbumin, enzyme rate method for testing β-hydroxybutyric aeidand and dry chemical method in urine automatic analyzer for determination of urine ketone on healthy control group, type-2 diabetes mellitus group （T2MD） , and diabetic nephropathy group （DN） , and the above results were analyzed. Results The exhale acetone level in T2MD and DN groups were higher than those in control group （P 〈 0. 01 ） ; In DN group, the level of exhale acetone with urinary microalbumin had a linear correlation. Conclusion The detection of exhale acetone is helpful to early diagnosis in diabetic nephropathy, also may reflect the renal injury promptly.

关 键 词：呼气丙酮 糖尿病肾病 气相色谱-质谱 尿微量白蛋白 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]