超氧化物歧化酶、丙二醛和内皮素在大鼠压疮模型损伤中的表达及作用  

Expression and Role of Superoxide Dismutase,Malondialdehyde and Endothelin in Rat Model of Pressure Ulcer Injury

作　　者：兰园淞 高文[1] 廖海涛[1] 杨立会[1] 韦义萍[1] LAN Yuan-song GAO Wen LIAO Hai-tao YANG Li-hui WEI Yi-ping(School of Nursing, Guangxi Medical University, Nanning, 530021, China)

机构地区：[1]广西医科大学护理学院,广西南宁530021

出　　处：《护理学报》2017年第9期1-6,共6页Journal of Nursing（China）

基　　金：广西教育厅广西高校科学技术研究项目(KY2015YB072);广西教育厅研究生创新项目(YCSZ2015123)

摘　　要：目的通过压力装置建立大鼠压疮模型,检测模型中的超氧化物歧化酶、丙二醛和内皮素的水平,探讨指标与压疮模型损伤的相关性,为临床压疮的预防提供早期预测指标。方法将40只SD大鼠随机分成4组:空白组和2、3、4个缺血再灌注循环组,每组10只,通过施加和移除压力装置模拟缺血再灌注的方式建立压疮模型;用ELISA试剂检测血液中超氧化物歧化酶、丙二醛和内皮素的水平,按照光镜下皮肤肌肉损伤分级标准再将大鼠分成正常、轻度损伤、中度损伤和重度损伤,分析超氧化物歧化酶、丙二醛和内皮素与压疮模型损伤程度的相关性。结果压疮模型缺血再灌注循环次数越多,大鼠皮肤肌肉组织损伤程度越严重,各缺血再灌注循环组损伤程度均重于空白组,差异有统计学意义(P<0.01);随着损伤程度的加重,氧化物歧化酶含量降低,丙二醛、内皮素含量增加,轻度、中度、重度损伤大鼠的超氧化物歧化酶水平均低于正常组,而丙二醛和内皮素水平均高于空白组,各组组间比较,差异均具有统计学意义(P<0.01);损伤严重程度与超氧化物歧化酶呈密切负相关(r=-0.930,P<0.01),与丙二醛呈密切正相关(r=0.924,P<0.01),与内皮素呈密切正相关(r=0.927,P<0.01)。结论超氧化物歧化酶含量的下降,丙二醛和内皮素含量增加是组织损伤加重的影响因素,结合生化指标检测对压疮发生早期的评估会更加具有预见性。Objective To detect the levels of superoxide dismutase, malondialdehyde and endothelin in the blood of rats, and study the correlation between them and the injury of pressure ulcer model, to provide early prediction index for the occurrence of clinical pressure sores. Methods Forty SD rats were randomly divided into four groups： blank group, ischemia reperfusion group 2,3,4, with10 rats in each group. The model of pressure ulcer was established by applying and removing the pressure device; ELISA reagent was used to detect superoxide dismutase, malondialdehyde and endothelium in the blood; in accordance with the light microscope of skin and muscle injury grade, then rats were divided into normal ones, with mild injury, moderate injury and severe injury respectively.The relationship of superoxide dismutase, malondialdehyde and endothelin with the degree of the pressure ulcer damage model was analyzed. Results As pressure ulcer model ischemia reperfusion cycles increased, rat skin and muscle tissue damage were more serious and the injury degree in ischemia reperfusion groups was more serious than that in blank group. The difference was statistically significant（P〈0.01）. When the damage degree increased, the content of superoxide dismutase decreased but that of malondialdehyde and endothelium increased. The content of superoxide dismutase in mild, moderate and severe injury group was lower than that in blank group, but the content of malondialdehyde and endothelium was higher than that in blank group and intergroup comparison indicated statistical significance（P〈0.01）; The severity of injury was negatively correlated with superoxide dismutase（r=-0.930, P〈0.01）, positively with malondialdehyde（r=0.924, P〈0.01）, and endothelium（r=0.927,P〈0.01）. Conclusion The decrease of superoxide dismutase content and the increase of malondialdehyde and endothelium content are the influencing factor of tissue injury. The evaluation of early stage of pressure ulcer with biochemical indexes detection wil

关 键 词：缺血再灌注 压疮模型 超氧化物歧化酶 丙二醛 内皮素 

分 类 号：R47[医药卫生—护理学]