烤烟品种的多重PCR技术标记鉴别  

Tag identification of flue-cured tobacco varieties by multiplex PCR technique

作　　者：贾梦珠 孙九喆 苏东赢 杨金初 王二彬 许衡 王永超[1] 王君婷[1] 孟丹丹[1] 马林[1] 

机构地区：[1]郑州轻工业学院烟草科学与工程学院,河南郑州450001 [2]河南中烟工业有限责任公司技术中心,河南郑州450000

出　　处：《轻工学报》2017年第3期51-57,共7页Journal of Light Industry

基　　金：河南中烟有限责任公司科技项目(ZW2015012)

摘　　要：以12份烤烟品种为模板,针对6对SCAR引物进行引物比例和退火温度的条件优化,采用多重PCR技术,建立了12份烤烟品种的多重PCR指纹图谱和鉴别路线.结果表明,同普通PCR相比,多重PCR具有高效、快速、节约时间、成本低的优点,6对SCAR引物稳定性好、条带特异性强,能准确区分12份烤烟品种.With 12 varieties of flue-cured tobacco as a template, the conditions for primer concentration and annealing temperature for 6 pairs of SCAR primers were optimized. Multiplex PCR fingerprints and identifica-tion routes of 12 flue-cured tobacco varieties were established by multiplex PCR. The results show^ed that mul-tiplex PCR had the advantages of high efficiency , high speed , time saving and low^ cost compared with ordinary PCR. Six pairs of SCAR primers had good stability and specific band specificityflue-cured tobacco varieties accurately.

关 键 词：多重PCR技术 SCAR标记 烤烟品种鉴别 指纹图谱 

分 类 号：TS424[农业科学—烟草工业]