Bcl-2和PCNA表达在大鼠成肌细胞氧化应激损伤中的作用  

Effect of Bcl-2 and PCNA expression in rat myoblast injury induced by hydrogen peroxide

作　　者：毛挺挺 郑亚华 方红波 谢鲁吉 张伟丹 姜丽萍[2] 

机构地区：[1]宁波市医疗中心李惠利东部医院,浙江宁波325000 [2]上海交通大学医学院附属新华医院,上海200092

出　　处：《中国病理生理杂志》2017年第5期935-938,943,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81372064);上海市教委护理高原学科建设资助项目

摘　　要：目的:探讨Bcl-2、PCNA等蛋白表达在成肌细胞氧化应激损伤中的意义。方法:将对数生长期成肌细胞进行分组,结合碱性成纤维细胞生长因子(bFGF)预处理2 h及过氧化氢处理,分为正常对照(control)组、单纯bFGF组、氧化应激组(H_2O_2组)和干预组(bFGF+H_2O_2组)。免疫组化检测Bcl-2和Bax阳性沉积颗粒;免疫荧光观察成肌细胞形态及Bcl-2和Bax荧光表达;Western blot检测Bcl-2、PCNA等蛋白表达情况。结果:免疫荧光及免疫组化结果显示,与氧化应激组比较,干预组Bcl-2表达增加,Bax表达减少;Western blot显示,干预组Bcl-2和PCNA水平增加,Bax水平降低。结论:氧化应激诱导的大鼠成肌细胞损伤参与肌细胞病理进程。Bcl-2和PCNA表达上调可减轻成肌细胞损伤程度。AIM： To explore the role of Bcl-2 and PCNA expression in the injury of rat myoblasts induced by hydrogen peroxide （H2O2）.METHODS： Rat myoblasts at growth phase were divided into 4 groups based on basic fibroblast growth factor （bFGF） and H2O2 levels：normal control group, bFGF group, model group （H2O2 group） and treatment group （bFGF＋H2O2 group）. The expression of Bcl-2 and Bax was observed by immunohistochemistry and fluorescence methods. The protein levels of Bax, Bcl-2 and PCNA were determined by Western blot.RESULTS： Compared with model group, both immunofuorescence and fluorescence in treatment group showed enhanced Bcl-2 and low expression of Bax. Furthermore, the results of Western blot showed up-regulated PCNA and Bcl-2 protein and decreased Bax expression in treatment group.CONCLUSION： Oxidative stress results in the pathologic changes of myoblasts, and the up-regulation of Bcl-2 and PCNA may attenuate myoblast injury.

关 键 词：深部组织损伤 氧化应激 L6成肌细胞 碱性成纤维细胞生长因子 

分 类 号：R363.21[医药卫生—病理学]