免疫组化法和qPCR技术检测FBP1在结直肠腺癌中的表达及临床意义  

Immunohistochemistry and qPCR to detect the expression of FBP1 in colorectal adenocarcinoma and its clinical significance

作　　者：刘家有[1] 邓世山[1] 朱剑军[1] 侯令密[2] 谢少利 黄波[3] 

机构地区：[1]川北医学院人体解剖学教研室 [2]川北医学院附属医院普外科 [3]川北医学院临床技能培训中心,四川南充637000

出　　处：《川北医学院学报》2017年第2期167-171,共5页Journal of North Sichuan Medical College

基　　金：四川省教育厅基金项目(13ZB0242);川北医学院科研发展计划项目(CBY12-A-QN01)

摘　　要：目的:探讨果糖-1,6-二磷酸酶1(fructose-1,6-bisphosphatase 1,FBP1)基因在结直肠腺癌的表达水平及其与临床病理特征的联系。方法:q PCR技术检测川北医学院附属医院2014年9月至2016年2月新鲜结直肠腺癌组织及癌旁正常组织46例中FBP1基因的表达水平。免疫组化方法检测川北医学院附属医院2015年7月至2016年6月结直肠腺癌及癌旁对照组织的蜡块标本30例中FBP1的表达及分布情况。结果:q PCR技术检测结果表明,FBP1基因在结直肠腺癌组织中的表达水平低于在癌旁正常组织中的表达水平(t=2.934,P=0.006);FBP1基因在结直肠腺癌组织中的表达水平随着结直肠腺癌肿瘤的恶性程度的增加(F=13.267,P=0.000)、Duke’s分期的增加(F=15.864,P=0.000)、远处淋巴结的转移(t=14.285,P=0.000)而降低;FBP1基因在男性患者的结直肠腺癌组织中的表达水平高于其在女性患者的表达水平,但差异无统计学意义(t=1.294,P=0.165)。免疫组化结果显示FBP1在结直肠腺癌组织中低表达甚至缺失,而在癌旁正常组织中高表达,二者差异具有统计学意义(χ~2=19.461,P=0.000)。结论:FBP1基因及蛋白质的低表达可能在结直肠腺癌的发生发展中有着重要的作用,是其潜在的治疗靶标。ObjectiveTo explore the expression of FBP1 in colorectal adenocarcinoma and its clinical significance. Methods：46 pairs of fresh human colorectal adenocarcinoma tissues and its adjacent normal tissues were collected from the Affiliated Hospital of North Sichuan Medical College from September 2014 to February 2016 Q uantitative RT-PCR was used to detect the expression level ofFBP1 mRNA in tissues. 30 specimens of human colorectal adenocarcinoma tissues and its adjacent normal tissues were collected fromthe Affiliated Hospital of North Sichuan Medical College from July 2015 to June 2016, immunohistochemical was used to exam the levelof FBP1. Results ： Quantitative RT-PCR show that the expression level of FBP1 mRNA in colorectal adenocarcinoma tissues was remarkablylower than that in adjacent normal colorectal tissues （t = 2. 934, P = 0. 006） . The expression level of FBP1 decreased as the increaseof malignancy degree and D uke, s stages and distant lymph metastases in colorectal adenocarcinoma （ P = 0. 000 ） . The expressionlevel of FBP1 mRNA in male patients was higher than that in those females, but the difference was not significant （ t = 1. 294, P =0. 165） . Immunohistochemical results show that the expession level of FBP1 in colorectal adenocarcinoma tissues was lower than that inadjacent normal colorectal tissues = 19. 461 ,P = 0. 0 00 ）. Conclusion：The decreased expression level of FBP1 mRNA and its proteinmay act a critical role in colorectal adenocarcinoma and may be used as a potential therapeutic target for colorectal adenocarcinoma.

关 键 词：结直肠腺癌 果糖-1 6-二磷酸酶1 免疫组化法 qPCR 

分 类 号：R735.3[医药卫生—肿瘤]