转移相关基因2对喉癌细胞系Hep-2的增殖、迁移和侵袭的影响  

Effect of metastasis associated gene 2 on proliferation,migration and invasion of laryngeal carcinoma cell line Hep-2

作　　者：马鹏[1] 冯俊[1] 杜经纬[1] 

机构地区：[1]川北医学院第二临床医学院.南充市中心医院耳鼻咽喉头颈外科,四川南充637000

出　　处：《川北医学院学报》2017年第2期172-174,共3页Journal of North Sichuan Medical College

基　　金：四川省教育厅项目(13ZB0241);川北医学院博士科研启动项目(CBY-QD-08)

摘　　要：目的:探讨转移相关基因2(MTA2)对喉癌细胞系Hep-2的增殖、迁移和侵袭的影响。方法:通过针对MTA2基因的小干扰RNA(siRNA-MTA2)对MTA2基因表达进行下调,采用RT-PCR和Transwell检测转染,作为实验组,对照组转染阴性对照(sicontrol),通过MTT、细胞划痕实验和Transwell法分别检测MTA2对Hep-2的增殖、迁移和侵袭的影响。结果:实验组MTA2mRNA和蛋白表达水平明显低于对照组(P<0.05),实验组培养7d和9d时细胞增殖能力明显低于对照组(P<0.05),实验组细胞迁移能力和细胞侵袭能力明显低于对照组(P<0.05)。结论:MTA2与喉癌细胞系Hep-2细胞增殖、迁移和侵袭密切相关,有望成为治疗喉癌的靶点。Objective To investigate the effect of metastasis associated gene 2 （ MTA2） on the proliferation, migration and invasionof laryngeal carcinoma cell line Hep-2. Methods ：MTA2 gene expression was down regulated by small interfering RNA （ siRNAMTA2）of MTA2 gene,and the transfection was detected by RT-PCR and Transwell. As the experimental group,the control group wastransfected with negative control （sicontrol） ,and the effect of MTA2 on the proliferation, migration and invasion of Hep-2 was detectedby M TT,cell scratch test and Transwell assay. Results：the expression level of MTA2mRNA and protein in the experimental group wassignificantly lower than that in the control group （P 〈 0 .0 5 ）. The cell proliferation ability of the experimental group was significantlylower than that of the control group （P 〈 0. 05） when cultured in 7d and 9D ,The cell migration ability and invasion ability of the experimentalgroup were significantly lower than those of the control group （P 〈 0. 0 5 ）. Conclusion： MTA2 is closely related to the proliferation,migration and invasion of Hep-2 cell line,w hich is expected to be a target for the treatment of laryngeal cancer.

关 键 词：MTA2 喉癌细胞 HEP-2 增殖 迁移 

分 类 号：R739.65[医药卫生—肿瘤]