缺氧诱导因子-2α对肝癌干细胞标记物EpCAM和CD133表达的影响  

Effects of hypoxia inducible factor-2 alpha on the expression of liver cancer stem cell marker EpCAM and CD133

作　　者：刘慧敏 马薇[2] 刘平[3] 金涛[2] 

机构地区：[1]武船中心医院,湖北武汉430000 [2]华中科技大学同济医学院附属梨园医院肿瘤科,湖北武汉430077 [3]华中科技大学同济医学院附属梨园医院骨科,湖北武汉430077

出　　处：《现代肿瘤医学》2017年第12期1866-1869,共4页Journal of Modern Oncology

摘　　要：目的:研究缺氧诱导因子-2α(HIF-2α)对肝癌干细胞标记物EpGAM和CD133表达的影响。方法:分别上调和下调肝癌细胞系HepG2细胞和Hep3B细胞中HIF-2α的表达,Real-time PCR检测EpGAM和CD133 mRNA表达的变化;Western Blot检测EpGAM和CD133蛋白水平的变化。结果:转染HIF-2α表达质粒后HepG2细胞和Hep3B细胞中EpGAM和CD133表达水平升高(P<0.05),干扰HIF-2α表达后肝癌细胞中EpGAM和CD133表达水平降低(P<0.05)。结论:HIF-2α可能在肝癌细胞"干性"维持方面发挥着积极的作用。Objective： To investigate the influence of hypoxia inducible factor-2 alpha（ HIF-2α） on the expression of liver cancer stem cell marker EpCAM and CD133. Methods： Up and down-regulation of the expression of HIF-2α in HepG2 cells and Hep3 B cells,respectively. Real-time P CR and Western Blot were used to detect the levels of EpCAM and CD133 mRNA and protein expression. Results： The expression of EpCAM and CD133 mRNA and protein was increased after the HIF-2α expression plasmids transfection（ P 〈0. 05）,and was decreased after HIF-2α shRNA plasmids transfection（ P〈 0. 05）. Conclusion： HIF-2α may play a positive role in maintaining the stemness of liver cancer cells.

关 键 词：HIF-2α 肝癌 EPCAM CD133 

分 类 号：R735.7[医药卫生—肿瘤]