IGF-1对胃癌血红素氧合酶-1表达的影响  

IGF-1 regulates the expression of HO-1 in gastric cancer

作　　者：蒋洁[1] 宁亚利[1] 李东辉[1] 宗伟[2] 白俊[1] 

机构地区：[1]陕西省人民医院肿瘤内科,陕西西安710068 [2]陕西省人民医院消化内科,陕西西安710068

出　　处：《现代肿瘤医学》2017年第12期1945-1948,共4页Journal of Modern Oncology

基　　金：陕西省自然科学基金资助项目(编号:2014JM4141)

摘　　要：目的:研究胃癌患者血清中胰岛素样生长因子-1(IGF-1)和血红素氧合酶-1(HO-1)的表达相关性,并探讨IGF-1对胃癌细胞中HO-1表达的调控作用。方法:收集胃癌患者血清标本,ELISA检测血清中IGF-1和HO-1的含量;胃癌细胞株SGC7901作为体外实验载体,不同浓度IGF-1干预胃癌细胞,Real time PCR和Western blot检测胃癌细胞中HO-1 mRNA和蛋白的表达量;随后在胃癌细胞培养基中加入IGF-1阻断剂,观察应用阻断剂前后胃癌细胞HO-1表达的变化。结果:胃癌患者血清中IGF-1和HO-1的表达较正常人群明显升高,且两者的表达呈正相关性。体外培养胃癌细胞SGC7901,IGF-1可显著促进胃癌细胞HO-1的表达,且该效应呈浓度依赖性。培养基中应用IGF-1阻断剂后,胃癌细胞HO-1的表达量明显受到抑制。结论:本实验探讨胃癌中IGF-1和HO-1表达的相关性,初步阐述IGF-1对胃癌HO-1表达的正向调控作用,为HO-1参与IGF-1促进胃癌发生及发展提供了理论依据。Objective：To investigate the relationship between the expressions of insul in - 1 ike growth factor - 1 （IGF - 1） and hemeoxygenase - 1 （ HO - 1） in patients with gastric cancer. To study the effects of IGF- 1on the ex-pression of HO - 1 in gastric cancer cells. Methods：We col lect serum samples of patients with gastric cancer , then , ELISA was used to detect the levels of IGF - 1 and HO - 1 expression. Gastric cancer cells SGC7901 was used in vitro,which were intervened by IGF - 1 in different concentrations. The mRNA and protein expression of HO - 1 in gastric cancer cells were detected by RT - PCR and Western blot analysis. Then the IGF - 1 blockers were added to the culture medium of gastric cancer cells,to observe the change of the expression of HO - 1 in gastric cancer cells both before and after using blockers. Results： The expressions o f IGF - 1 and HO - 1 in the patients w ith gastric canc-er significantly were increased than those in the controls. The significant positive correlations were observed between the levels of IGF - 1 and HO - 1. Gastric cancer cells SGC7901 were cultured in vitro,and the HO - 1 expression in gastric cancer cells was significantly enhanced by IGF - 1 by a concentration - dependent manner. The HO - 1 expres-sion in gastric cancer cells was significantly inhibited by IGF - 1 blockers. Conclusion： This experiment studies the correlation of IGF - 1 and HO - 1 in gastric cancer,and suggests the positive regulatory effects of IGF - 1on HO - 1 expression in gastric cancer cells. Our research provides the theoretical basis for gastric cancer.

关 键 词：胃癌 IGF-1 HO-1 

分 类 号：R735.2[医药卫生—肿瘤]