HMGB1和相关炎症因子在小鼠肝纤维化模型中的表达及意义  

Expression And Significance Of HMGB1 And Related Inflammatory Cytokines In Liver Fibrosis Animal

作　　者：邢媛 张芸[2] 刘明社[3] 赵中夫[2] Xing Y uan Zhang Y un Liu M ingshe et al(Zhenru Community Health Service Center Putuo District 9Shangha)

机构地区：[1]上海市普陀区真如社区卫生服务中心,200033 [2]长治医学院附属和平医院感染科 [3]长治医学院寄生虫学教研室

出　　处：《长治医学院学报》2017年第2期85-88,共4页Journal of Changzhi Medical College

基　　金：山西省自然科学基金(2008011073-4)

摘　　要：目的:探讨HMGB1、IL-6及TNF-α在CCL4导致的小鼠肝纤维化模型中的表达及意义。方法:昆明种雄性小鼠30只,采用CCL4灌胃法制备肝纤维化模型组(1μL/g CCL4油溶液灌胃,1次/5d),另设正常对照组10只,(1μL/g生理盐水灌胃,1次/5d)。在建模2周、4周、6周后留取血清及肝组织,ELISA法检测血清中透明质酸(HA)、Ⅰ型胶原蛋白(Col-Ⅰ)、Ⅲ型前胶原蛋白(PⅢP)和肿瘤坏死因子α(TNF-α)、白介素6(IL-6)的含量;Western blot法检测肝组织HMGB1的表达水平。结果:ELISA结果显示正常对照组血清HA、Col-Ⅰ、PⅢP和IL-6、TNF-α含量较少;正常组与模型组2周组、2周组与4周组、4周组与6周模型组血清HA、Col-Ⅰ、PⅢP和IL-6、TNF-α含量均较前组明显增加(P<0.05)。Western-blot结果显示正常对照组肝组织内无HMGB1表达,肝纤维化2周、4周、6周模型组与正常对照组比较,肝组织中HMGB1的表达明显增加,而且随着CCL4损伤时间的延长而逐渐升高(P<0.05)。结论:血清HMGB1和相关炎症因子IL-6、TNF-α的表达在CCL4导致的小鼠肝纤维化动物模型中明显增高。Objective:To explore the expression and significance of HMGB1,IL-6and TNF-αin the mice models of CCL4 induced liver fibrosis.Methods:Thirty Kunming male mice were modeled liver fibrosis by the ccl4 gavage preparation(1μL/g saline was administered every five days),and 10 mice in the normal control group.Serum and liver tissue were taken at 2weeks,4weeks,6weeks of modeling.The parameters of plasma HA,Col-Ⅰ,PⅢP and IL-6,TNF-αwere measured by ELISA.The level of HMGB1 in liver tissue was measured by western blot.Results:The levels of HA、Col-Ⅰ、PⅢP and IL-6,TNF-αin the normal group were very low in serum measured by ELISA.Compared with the former group,the levels of them in normal group vs 2weeks,2weeks vs 4weeks and 4weeks vs 6weeks model group were remarkably increased(P<0.05).Western-blot results showed that no HMGB1 expression in the liver tissue of normal control group.Compared with the normal control group,the levels of HMGB1 in 2weeks,4weeks and 6weeks model group were remarkably increased,and the HMGB1 gradually increased with the CCL4 damage time(P<0.05).Conclusion:In the CCL4 induced liver fibrosis model,the levels of HMGB1 and inflammatoryfactorsIL-6,TNFαare significantly increased.

关 键 词：高迁移率族蛋白B1 慢性炎症 炎症因子 肝纤维化因子 

分 类 号：R363[医药卫生—病理学]