HPLC法同时测定复方参芎滴丸中4种成分的含量  

Simultaneous determination of four components in Compound Shenxiong pills by HPLC

作　　者：罗宏稳 谢淑桐 毋福海[3] 范国荣 

机构地区：[1]广东药科大学中药学院,上海200433 [2]广东药科大学药学院,上海200433 [3]广东药科大学公共卫生学院,广东广州510006 [4]上海市药物(中药)代谢产物研究重点实验室,上海200433

出　　处：《广东药科大学学报》2017年第2期191-194,共4页Journal of Guangdong Pharmaceutical University

基　　金：上海市药物(中药)代谢产物研究重点实验室开放课题(2015SHDX1001)

摘　　要：目的建立同时测定复方参芎滴丸中丹参素钠、丹酚酸B、丹参酮ⅡA和阿魏酸4种成分质量分数的HPLC法。方法采用Welchrom-C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相为体积分数0.1%甲酸水溶液-甲醇-乙腈(体积比40∶60),梯度洗脱;流量为1.0 mL/min;检测波长为286 nm;柱温为25℃。结果丹参素钠、阿魏酸、丹酚酸B和丹参酮ⅡA分别在1.92~30.7μg/mL(r=0.999 4)、1.12~18.0μg/mL(r=0.999 1)、3.50~56.0μg/mL(r=0.999 4)、4.25~68.0μg/mL(r=0.9997)范围内呈良好的线性关系,平均回收率分别为104.4%、101.0%、99.6%、96.4%,RSD值分别为1.955%、2.520%、2.154%、1.458%。结论该方法简便、精确、重复性好,适用于复方参芎滴丸中4种成分的测定。Objective To establish the HPLC method for simultaneous determination of salvianic acid A sodium, salvianolic acid B, tanshinone ⅡA and ferulic acid in Compound Shenxiong pills. Methods The analysis was performed on Welchrom-C18 column （4.6 mm×250 mm, 5 μm）, with mobile phase of 0.1% formic acid aqueous solution （A） and acetonitrile-methanol （40：60） （B） for gradient elution, and the flow rate was 1.0 mL/min. The detection wavelength was set at 286 nm, and column temperature was maintained at 25℃. Results Salvianic acid A sodium, ferulie acid, salvianolic acid B and tanshinone Ⅱ A showed good linear relationships within the range of 1.92-30.7 μg/mL （r= 0.999 4）, 1.12-18.0 μg/mL （r= 0,999 1）, 3.50-56.0 μg/mL （r=0.999 4）, and 4.25-68.0 μg/mL （r= 0.999 7）, with average recoveries of 104.4% （ RSD= 1.955%）, 101.0% （ RSD = 2.520%）, 99.6% （ RSD = 2. 154%）, and 96.4% （ RSD = 1.458%）, respectively. Conclusion This simple, accurate and reproducible method is suitable for the determination of these four constituents in Compound Shenxiong pills.

关 键 词：复方参芎滴丸 丹参素钠 阿魏酸 丹酚酸B 丹参酮ⅡA 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] R286.0[理学—化学]